Ke Li
1*
Huan Li
1
Weiling Liang
1*
Li Hu Wang
1*The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Bioinformatics
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1511713
This article is part of the Research Topic Multi-omics and Computational Biology in Horticultural Plants: From Genotype to Phenotype, Volume III View all articles
Identification of the AHP Family Reveals Their Critical Response to Cytokinin Regulation During Adventitious Root Formation in Apple Rootstock
Provisionally accepted- 1 Hebei University of Engineering, Handan, China
- 2 Northwest A&F University, Xianyang, Shaanxi Province, China
Adventitious root (AR) formation is a bottleneck for vegetative proliferation. In this study, 13 AHP genes (MdAHPs) were identified in the apple genome. Phylogenetic analysis grouped them into 3 clusters (I, II, III), with 4, 4, and 5 genes respectively. The 13 MdAHPs family members were named MdAHP1 to MdAHP13 by chromosome positions. The physicochemical properties, phylogenetic relationship, motifs, and elements of their proteins were also analyzed. The amino acid quantity varied from 60~189 aa, isoelectric point lay between 4.10 and 8.93, and there were 3~7 protein-conserving motifs. Excluding MdAHP6, other members' promoter sequences behaved 2-4 CTK response elements.Additionally, the expression characteristics of MdAHPs family members at key stages of AR formation and in different tissues were also examined with exogenous 6-BA and Lov treatments. The results showed that MdAHP3 might be a key member in AR formation. GUS staining indicated that the activity of the MdAHP3 promoter was also significantly enhanced by CTK treatment. The protein interactions of MdAHP3/MdAHP1 and MdAHP3/MdAHP6 were verified. Compared with WT, 35S::MdAHP3 transgenic poplars inhibited AR formation. The above experimental results suggested that MdAHP3, as a key family member, interacts with MdAHP1 and MdAHP6 proteins to jointly mediate AR formation in apple rootstocks.
Keywords: apple, Adventitous root, Cytokinin, MdAHP, 35S::MdAHP3 transgenic plants
Received: 15 Oct 2024; Accepted: 18 Dec 2024.
Copyright: © 2024 Li, Li, Liang, Liu, Tian, Wang and Wei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Ke Li, Hebei University of Engineering, Handan, China
Weiling Liang, Hebei University of Engineering, Handan, China
Jing Ju Liu, Hebei University of Engineering, Handan, China
Hui Yue Tian, Northwest A&F University, Xianyang, 712100, Shaanxi Province, China
Li Hu Wang, Hebei University of Engineering, Handan, China
Yan Hong Wei, Hebei University of Engineering, Handan, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.