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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Symbiotic Interactions
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1507307
This article is part of the Research Topic Plant-microbes Interactions and Resistance Against Abiotic Stress View all 10 articles

GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max)

Provisionally accepted
Jinhui Wang Jinhui Wang 1*Mingliang Yang Mingliang Yang 1Chengjun Lei Chengjun Lei 1Chao Ma Chao Ma 1Xiuming Hou Xiuming Hou 1Mingming Yao Mingming Yao 1Liang Mi Liang Mi 1Enliang Liu Enliang Liu 2Linli Xu Linli Xu 2Shukun Wang Shukun Wang 1Chang Xu Chang Xu 1Chunyan Liu Chunyan Liu 1QINGSHAN CHEN QINGSHAN CHEN 1Dawei XIN Dawei XIN 1
  • 1 Northeast Agricultural University, Harbin, China
  • 2 Xinjiang Academy of Agricultural Sciences, Urumqi, China

The final, formatted version of the article will be published soon.

    Brassinosteroids (BRs) are key phytohormones influencing soybean development, yet their role in symbiosis remains unclear. Here, a RNA-Seq analysis was conducted in which BR signaling was found to suppress nodule formation and many DEGs enriched in immunity-related pathways. WGCNA analyses led to the identification of GmWRKY33a as being responsive to BR signaling in the context of symbiosis establishment. Transgenic hairy roots analyses indicated that GmWRKY33a served as a negative regulator of the establishment of symbiosis. The qRT-PCR analysis confirmed that BR signaling upregulates GmWRKY33a, leading to nodulation suppression and activation of soybean immune responses. In summary, our research revealed that BR suppresses root nodule formation by modulating the immune signaling pathway in soybean roots. We further identified that GmWRKY33a, a crucial transcription factor in BR signaling, plays a negative role in the symbiotic establishment.

    Keywords: Soybean, Symbiosis, Brassinosteroids (BRs), RNA-Seq, WGCNA, GmWRKY33a

    Received: 07 Oct 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Wang, Yang, Lei, Ma, Hou, Yao, Mi, Liu, Xu, Wang, Xu, Liu, CHEN and XIN. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jinhui Wang, Northeast Agricultural University, Harbin, China

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