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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1501661

Development of new powdery mildew resistant lines in garden pea (Pisum sativum L.) using induced mutagenesis and validation of resistance for er1 and er2 gene through molecular markers

Provisionally accepted
Akhilesh Sharma Akhilesh Sharma 1*Devinder Kumar Banyal Devinder Kumar Banyal 1Vinod Janardan Dhole Vinod Janardan Dhole 2Bansuli . Bansuli . 1Ranbir Singh Rana Ranbir Singh Rana 1Rajesh Kumar Rajesh Kumar 3Prabhat Kumar Prabhat Kumar 4Nimit Kumar Nimit Kumar 1Srishti . Srishti . 1Arshia Prashar Arshia Prashar 1Vivek Singh Vivek Singh 1Anoushka Sharma Anoushka Sharma 1
  • 1 Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, India
  • 2 Bhabha Atomic Research Centre (BARC), Mumbai, Maharashtra, India
  • 3 Indian Institute of Vegetable Research (ICAR), Varanasi, Uttar Pradesh, India
  • 4 Ministry of Agriculture & Farmers Welfare, New Delhi, Delhi, India

The final, formatted version of the article will be published soon.

    Powdery mildew (PM) caused by Erysiphie pisi Syd. is the most devastating disease of pea, affecting fresh pea production as well as quality of the marketable harvest worldwide. The efforts were made to develop PM resistant mutants of popular pea varieties 'Lincoln' and 'Azad P-1' through induced mutations by following gamma irradiation (300, 400, 500, and 600 Gy) and chemical mutagenesis i.e. ethyl methane sulfonate (EMS) (0.3% and 0.4%). The screening of 13868 M2 progenies at Kukumseri (summer season) followed by M3 generation at Palampur (winter season) resulted in the isolation of six putative PM resistant mutants. The rigorous evaluation of these progenies under in vivo (filed screening) and in vitro (artificial screening under greenhouse and detached leaf assay method) conditions over the years resulted in the isolation of three PM resistant mutants viz., L-40-1014, L-0.3-139, and AP-0.3-129. SSR markers 'PSMPSAD60 d' and 'PSMPA5 c' linked to the er-1 gene indicated the presence of 'er1' gene in the mutant L-0.3-139 while er-2 gene linked SCAR marker 'ScX171400' and SSR marker 'AD141' indicated the probability of 'er-2' gene in mutant L-40-1014. The known markers linked to PM resistance genes could not be validated in the mutant AP-0.3-129, suggested to identify new markers linked to PM resistance. These PM resistant mutants can be the promising candidates as the new source of resistance for future pea breeding programme.

    Keywords: Mutagenesis, Garden pea, Erysiphe pisi Syd, Resistance, screening, er genes, Molecular markers

    Received: 25 Sep 2024; Accepted: 31 Dec 2024.

    Copyright: © 2024 Sharma, Banyal, Dhole, ., Rana, Kumar, Kumar, Kumar, ., Prashar, Singh and Sharma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Akhilesh Sharma, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, India

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