Skip to main content

ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Genetics, Epigenetics and Chromosome Biology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1501642
This article is part of the Research Topic Innovative Technologies of Molecular Cytogenetics and The Application in Plant Biology View all articles

Frequent variations and phylogenetic relationships within the genus Secale identified by ND-FISH according to the genome-wide universal oligonucleotides chromosome probes

Provisionally accepted
Zhi Li Zhi Li Zixin Sun Zixin Sun Tianheng Ren Tianheng Ren *
  • Sichuan Agricultural University, Ya'an, China

The final, formatted version of the article will be published soon.

    Introduction: Rye (Secale cereale L.) played a very important role in wheat genetic improvement and forage production worldwide. However, since rye is a kind of cross-pollinated plant, high levels of genetic heterozygosity and heterogeneity existed in the genome. Genome-wide variation in repeat sequences is one of the most important reasons for chromosome evolution in rye. High-precision cytological identification can effectively identify the heterochromatin or repeat sequence variations in the rye genome, and the relationship between different rye varieties can be identified while obtaining the FISH-karyotype of different rye varieties. The evolution of rye chromosomes can be analyzed by the variation degree of different probes on rye chromosomes. Methods: All materials were identified by non-denaturing fluorescence in situ hybridization (ND-FISH). Five probes, (AAC)6, Oligo-pSc119.2-1, Oligo-pTa71A-2, Oligo-pSc200, and Oligo-pSc250 were used to identify rye chromosomes. Results: 15 rye varieties including S. cereale (cultivated rye and weedy rye), S. strictum (wild rye), S. sylvestre (wild rye), and S. vavilovii (wild rye) were examined by five oligonucleotides probes. 92 signal sites and 2074 signal patterns were observed, suggesting that high polymorphisms exist in the different rye genomes. The karyotypes of 15 rye varieties were obtained, the frequency of different signal types at each signal site was calculated and the model diagrams of probes (AAC)6, Oligo-pSc119.2-1, Oligo-pTa71A-2, Oligo-pSc200 + Oligo-pSc250 were drawn. The results showed that the rate of variation of different chromosomes of rye was not consistent. 1R, 6R, and 7R have higher variation and genetic diversity, while 2R and 3R have lower variation and are more conserved relative to other chromosomes. The results also indicated that S. sylvestre has a far genetic distance from other rye species, and S. vavilovii might be one of the ancestors of Chinese rye varieties. Discussion: Results from this study confirmed rapid chromosome change and high levels of chromosome diversity in rye.

    Keywords: Rye, fish, genetic diversity, Oligonucleotides, evolution

    Received: 26 Sep 2024; Accepted: 22 Nov 2024.

    Copyright: © 2024 Li, Sun and Ren. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Tianheng Ren, Sichuan Agricultural University, Ya'an, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.