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MINI REVIEW article

Front. Plant Sci.
Sec. Plant Biotechnology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1501092
This article is part of the Research Topic Engineering Future Crops Through Genome Editing View all 7 articles

CRISPR/Cas9-based mutant library screening: the discovery of novel genes regulating immune responses in cotton and rice

Provisionally accepted
Sang Ryeol Park Sang Ryeol Park Seungmin Son Seungmin Son *
  • Rural Development Administration (South Korea), Jeonju, Republic of Korea

The final, formatted version of the article will be published soon.

    The environmental conditions play a crucial role in determining crop yield, which is essential for ensuring food and nutritional security. However, rapid climate change is exacerbating global environmental stress, leading to severe biotic pressures on crops. Therefore, enhancing crop resilience to pathogens has become one of the most pressing challenges for humanity.Large-scale mutant library screening is the most efficient strategy for identifying numerous genes associated with specific traits. The revolutionary CRISPR/Cas9 system has ushered in a new era in the construction of mutant library. However, its application in crop plants has been relatively scarce compared to mammals, largely due to challenges in accessibility. Fortunately, several research groups have recently developed CRISPR/Cas9-based mutant libraries, successfully identifying a variety of genes involved in crop immunity. In this review, we present an overview and discussion of studies that have generated significant results through the use of CRISPR/Cas9 library screening to identify novel genes associated with resistance to biotic stresses within the field of plant research.

    Keywords: CRISPR/Cas9, crop, Genetic resources, Mutant library screening, new breeding technology, Plant Immunity

    Received: 24 Sep 2024; Accepted: 28 Oct 2024.

    Copyright: © 2024 Park and Son. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Seungmin Son, Rural Development Administration (South Korea), Jeonju, Republic of Korea

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.