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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Bioinformatics
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1496351

Genome-wide analysis of Nicotiana tabacum IDD genes identifies NtIDD9 as a regulator of leaf angle

Provisionally accepted
Zefeng Li Zefeng Li 1Peijian Cao Peijian Cao 1Huabing Liu Huabing Liu 2Jianfeng Zhang Jianfeng Zhang 1Zhaopeng Luo Zhaopeng Luo 1Hui Zhang Hui Zhang 1Mingzhu Wu Mingzhu Wu 1Xiaodong Xie Xiaodong Xie 1*
  • 1 Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China
  • 2 China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    INDETERMINATE DOMAIN (IDD) gene family, encoding a class of C2H2 transcription factor, played diverse roles in land plants. IDD family in tobacco (Nicotiana tabacum) has not been characterized. In this study, 26 NtIDDs were identified in tobacco genome. Phylogenetic analysis showed NtIDDs were divided into five groups. Motif analysis revealed ID domain was conserved in NtIDDs. Gene duplication analysis demonstrated segmental/whole genome duplication and dispersed duplication would have occurred in NtIDDs. Cis-element analysis predicted hormone, stress and development-related elements located in NtIDD promoters. Expression analysis revealed tissue preference patterns and differential hormone responses in NtIDDs. Further investigations on the function of NtIDD9 exhibited increased leaf angle degrees in RNA silencing plants. Cellular localization suggested NtIDD9 expressed in endodermis of leaf petiole base. Subcellular localization analysis revealed NtIDD9 protein was located in the nucleus. Hormone quantification found that the levels of auxin, ABA, JA and GA were significantly changed in NtIDD9 silenced plants. Thus, the study suggested NtIDD9 participated crucial role in modulation of leaf angle development. Overall, these findings lay foundations for future function and mechanism research on IDDs in tobacco.

    Keywords: IDD family, Evolutionary analysis, expression analysis, leaf angle, Nicotiana tabacum

    Received: 14 Sep 2024; Accepted: 03 Dec 2024.

    Copyright: © 2024 Li, Cao, Liu, Zhang, Luo, Zhang, Wu and Xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Xiaodong Xie, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China

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