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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Abiotic Stress
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1492854

Selection of suitable reference lncRNAs for gene expression analysis in Osmanthus fragrans under abiotic stresses, hormone treatments, and metal ion treatments

Provisionally accepted
Yingting Zhang Yingting Zhang 1,2,3Qingyu Yan Qingyu Yan 1*Hui Xia Hui Xia 4*Xiangling Zeng Xiangling Zeng 1,2,3*Jie Yang Jie Yang 1,2,3*Xuan Cai Xuan Cai 1,2,3*Zeqing Li Zeqing Li 1,2,3Hongguo Chen Hongguo Chen 1,2,3*Jing-Jing Zou Jing-Jing Zou 1,2,3*
  • 1 Hubei University of Science and Technology, Xianning, Hubei, China
  • 2 National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Xianning, China
  • 3 Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Xianning, China
  • 4 Central South University Forestry and Technology, Changsha, Hunan Province, China

The final, formatted version of the article will be published soon.

    Osmanthus fragrans, a well-regarded traditional flower in China, holds extensive applications in gardening, food, cosmetics, and traditional Chinese medicine. Despite its importance, research on long non-coding RNAs (lncRNAs) in O. fragrans has been constrained by the absence of reliable reference genes (RGs). To address this limitation, we employed five distinct algorithms, i.e., delta-Ct, NormFinder, geNorm, BestKeeper, and RefFinder, to evaluate the expression stability of 17 candidate RGs across various experimental conditions. The results indicated the most stable RG combinations under different conditions as follows: cold stress: lnc00249739 and lnc00042194;

    Keywords: Osmanthus fragrans, reference lncRNAs, Flowering stage, abiotic stress, Hormone treatment, metal ion treatment lnc00239991, lnc00042194, lnc00067193

    Received: 08 Sep 2024; Accepted: 26 Dec 2024.

    Copyright: © 2024 Zhang, Yan, Xia, Zeng, Yang, Cai, Li, Chen and Zou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Qingyu Yan, Hubei University of Science and Technology, Xianning, 050091, Hubei, China
    Hui Xia, Central South University Forestry and Technology, Changsha, 410000, Hunan Province, China
    Xiangling Zeng, Hubei University of Science and Technology, Xianning, 050091, Hubei, China
    Jie Yang, Hubei University of Science and Technology, Xianning, 050091, Hubei, China
    Xuan Cai, Hubei University of Science and Technology, Xianning, 050091, Hubei, China
    Hongguo Chen, Hubei University of Science and Technology, Xianning, 050091, Hubei, China
    Jing-Jing Zou, Hubei University of Science and Technology, Xianning, 050091, Hubei, China

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