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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Nutrition
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1489112
Synergistic Effects of Yeast and Plant Growth-Promoting Bacteria on Tobacco Growth and Soil-Borne Disease Suppression: evidence fromInsights from Pot and Field Experiments
Provisionally accepted
Kai Teng
1
Yu Zhou
2
Hui Mao
1
Sheng Zhang
1
Xianjun Long
1
Jingjing Ma
2
Delong Meng
3
Huaqun Yin
3*
Yunhua Xiao
2*- 1 Hunan Tobacco Company Xiangxi Autonomous Prefecture Corporation, Jishou City, China
- 2 Hunan Agricultural University, Changsha, Hunan, China
- 3 Central South University, Changsha, Hunan Province, China
Background: Tobacco (Nicotiana tabacum L.) is an important economic crop, and the use of plant growth-promoting bacteria (PGPB) to enhance its growth and suppress soil-borne diseases has garnered considerable research interest. However, the potential of yeast to augment the growth-promoting and disease-suppressing effects of PGPB on tobacco remains unclear.Methods: This study investigated the effects of Pichia sp. microbial fertilizer (J1), PGPB-Klebsiella oxytoca microbial fertilizer (ZS4), and their composite fertilizer (JZ) on tobacco growth indexes, soil properties, and soil microbial community through a pot experiment. Additionally, field experiments were conducted to further assess the efficacy of the composite microbial fertilizer on tobacco growth and the incidences of soil-borne diseases, including tobacco bacterial wilt (TBW) and tobacco black shank (TBS).In the pot experiment, application of the microbial fertilizers significantly enhanced soil organic matter (OM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), available phosphorus (AP), and available potassium (AK) levels. Compared to the control group (CK), J1, ZS4, and JZ microbial fertilizers significantly promoted tobacco growth, and the composite microbial fertilizers demonstrated superior to the individual microbial fertilizers. We found that the application of microbial fertilizer led to significant alterations in the structure and composition of the bacterial and fungal communities based on the high-throughput sequencing of 16S rRNA and internal transcribed spacer (ITS) regions. The bacterial and fungal diversity indexes showed a decreasing trend. Key microorganisms such as Sphingomonas, Kitasatospora, Nitrosospira, Mortierella, and Trichoderma were identified as influential in regulating soil physicochemical parameters to enhance tobacco growth.Functional prediction further demonstrated a significant increase in the relative abundances of certain enzymes, including Alkaline phosphatase, 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase), and Peroxidase, as well as antimicrobial substances like Tetracycline, Isoquinoline alkaloid, and Phenylpropanoids, following inoculation with the fertilizer. Besides, field experiments revealed that the JZ fertilizer significantly promoted tobacco growth and reduced the incidence of TBW and TBS, indicating its potential for further application in tobacco cultivation.
Keywords: Pichia sp., plant growth-promoting bacteria, Tobacco growth, soil Soil microbial community, soil Soil properties
Received: 31 Aug 2024; Accepted: 15 Oct 2024.
Copyright: © 2024 Teng, Zhou, Mao, Zhang, Long, Ma, Meng, Yin and Xiao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Huaqun Yin, Central South University, Changsha, 130012, Hunan Province, China
Yunhua Xiao, Hunan Agricultural University, Changsha, 410128, Hunan, China
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