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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Crop and Product Physiology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1488572
This article is part of the Research Topic Fruit Trees Under Stress: Physiological, Biochemical, and Molecular Mechanisms View all articles

Transcriptome analysis revealed that AcWRKY75 transcription factor reduced the resistance of kiwifruit to Pseudomonas syringae pv. Actinidiaeactinidiae

Provisionally accepted
Lixia Ye Lixia Ye 1Minmin Luo Minmin Luo 1*Yafang Wang Yafang Wang 1,2*Mengqi Yu Mengqi Yu 1,2*Zhi Wang Zhi Wang 1Fuxi Bai Fuxi Bai 1Xuan Luo Xuan Luo 1Li Li Li Li 3*Qiong Huang Qiong Huang 1Jue Peng Jue Peng 1*Qi Chen Qi Chen 4*Qinghong Chen Qinghong Chen 1*Lei Gao Lei Gao 1*Lei Zhang Lei Zhang 1*
  • 1 Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, China
  • 2 College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei Province, China
  • 3 Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences (CAS), Wuhan, Hubei Province, China
  • 4 Chibi Shenshan Xingnong Technology Co. LTD, Chibi, China

The final, formatted version of the article will be published soon.

    The kiwifruit canker disease caused by Pseudomonas syringae pv. Actinidiae actinidiae (Psa) seriously threatens the development of kiwifruit industry. So far, only a limited number of Psa-resistant kiwifruit varieties have been identified, and the underlying molecular mechanisms are still largely unknown. In this study, we evaluated the Psa resistance of six hybrid populations and screened a resistant segregation population R1F2. Then, transcriptome analysis on the Psa extremely high-resistant (HR) and extremely high-susceptible (HS) plants of the R1F2 population was performed. KEGG enrichment analysis revealed that differentially expressed genes (DEGs) were significantly enriched in plant hormone signal transduction pathways, including auxin, abscisic acid, zeatin, jasmonic acid and salicylic acid. Furthermore, several transcription factors (TFs), especially WRKY TFs, were identified among the DEGs.The qRT-PCR showed that AcWRKY75 was highly expressed in the HS plants.Additionally, AcWRKY75 was significantly induced in the HS cultivar 'Hongyang' after Psa inoculation. Sequence amplification analysis showed that there was polymorphism in the DNA sequence of AcWRKY75 gene, but no HR or HS-specific differences were observed. Subcellular localization and transcriptional activity analysis confirmed that AcWRKY75 functions as a nucleus-located transcriptional activator. Transient overexpression of AcWRKY75 in kiwifruit leaves reduced the resistance to Psa, while silencing AcWRKY75 by virus-induced gene silencing (VIGS) slightly enhanced the resistance to Psa. Furthermore, AcWRKY75 exhibited a weak interaction with the promoter of the ABA-related DEG AcBet V1 (Acc27163). Our findings elucidated that AcWRKY75 may negatively regulate the Psa resistance of kiwifruit through the hormone signaling pathway, which laid a foundation for the analysis of the disease resistance mechanism of kiwifruit canker.

    Keywords: Kiwifruit, Pseudomonas syringae pv. Actinidiaeactinidiae, WRKY75, Transcriptome, transcription factor, Disease Resistance

    Received: 30 Aug 2024; Accepted: 07 Oct 2024.

    Copyright: © 2024 Ye, Luo, Wang, Yu, Wang, Bai, Luo, Li, Huang, Peng, Chen, Chen, Gao and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Minmin Luo, Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, China
    Yafang Wang, Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, China
    Mengqi Yu, Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, China
    Li Li, Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences (CAS), Wuhan, 430074, Hubei Province, China
    Jue Peng, Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, China
    Qi Chen, Chibi Shenshan Xingnong Technology Co. LTD, Chibi, China
    Qinghong Chen, Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, China
    Lei Gao, Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, China
    Lei Zhang, Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, China

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