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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1487092
This article is part of the Research Topic Soybean Breeding for Abiotic Stress Tolerance: Towards Sustainable Agriculture View all 9 articles

Genome-wide identification and tissue expression pattern analysis of TPS gene family in soybean (Glycine max)

Provisionally accepted
Huanli Li Huanli Li *Xiaoling Zhang Xiaoling Zhang Qinli Yang Qinli Yang Xiaoxia Shangguan Xiaoxia Shangguan Yanbin Ma Yanbin Ma
  • Cotton Research Institute, Shanxi Agricultural University, Yuncheng, Shanxi Province, China

The final, formatted version of the article will be published soon.

    The terpene synthase (TPS) plays a pivotal roles in plant growth, development, and enhancing resilience against environmental stresses. Despite this, the bioinformatics analysis of the TPS family gene in soybean (Glycine max) is lacking. In this study, we investigated 36 GmTPS members in soybean, exhibiting a diverse range of protein lengths, spanning from 144 to 835 amino acids. A phylogenetic tree was constructed from these GmTPS genes revealed a classification into five distinct subgroups: Group1, Group2, Group3, Group4 and Group5. Notably, within each subgroup, we identified the motifs of GmTPS proteins were similar, although variations existed among different subfamilies. Gene duplication events analysis demonstrated that TPS genes expand differently in G. max, A. thaliana and O. sativa. Among, both tandem duplication and Whole genome duplication contributive to the expansion of TPS genes in G. max, and Whole genome duplication played a major role. Moreover, the cis-element analysis suggested that TPS is related to hormone signals, plant growth and development and environmental stress. Yeast two-hybrid (Y2H) assay results indicated TPS protein may form heterodimer to function, or may form complex with P450 proteins to function.RNA-seq results revealed a higher higher expression of most GmTPS genes in flowers, suggesting their potential contribution to flower development. Collectively, these findings offer a provide a holistic knowledge of the TPS gene family in soybean and will facilitate further characterization of TPSs effectively.

    Keywords: Glycine max, TPS gene family, phylogenetic analysis, duplicated events, RNA-Seq

    Received: 27 Aug 2024; Accepted: 09 Sep 2024.

    Copyright: © 2024 Li, Zhang, Yang, Shangguan and Ma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Huanli Li, Cotton Research Institute, Shanxi Agricultural University, Yuncheng, 030801, Shanxi Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.