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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Bioinformatics
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1484531
This article is part of the Research Topic Evolutionary Dynamics, Functional Variation and Application of Plant Organellar Genome View all 7 articles

Organelle Genome Assembly, Annotation, and Comparative Analyses of Typha latifolia and T. domingensis: Two Keystone Species for Wetlands Worldwide

Provisionally accepted
Thida Soe Thida Soe 1Jiali Kong Jiali Kong 1Liyun Nie Liyun Nie 1Jie Wang Jie Wang 1Dan Peng Dan Peng 1Zhiqiang Wu Zhiqiang Wu 2*Luke R Tembrock Luke R Tembrock 3*
  • 1 Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
  • 2 Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
  • 3 Department of Agricultural Biology, Colorado State University, Fort Collins, CO, United States, Colorado State, United States

The final, formatted version of the article will be published soon.

    Typha is a cosmopolitan aquatic plant genus that includes species with widespread global distributions. In previous studies, a revised molecular phylogeny was inferred using seven plastid loci from nine Typha species across different geographic regions.By utilizing complete organellar genomes, we aim to provide a more comprehensive dataset that offers a robust phylogenetic signal for resolving Typha species evolutionary relationships. Here, we assembled T. latifolia and T. domingensis mitochondrial genomes (mitogenomes) using a combination of short-read and long-read data (PacBio, ONT). we present the complete assembly and annotation of the organellar genomes of two common Typha species, T. latifolia and T. domingensis.The mitogenomes mitochondrial genomes (mitogenomes) of both species are assembled into single circular molecules of 395,136 bp and 395,140 bp in length, respectively, with a similar GC content of 46.7%. A total of 39 protein-coding genes, 17 tRNA genes, and 3 rRNA genes were annotated in both mitogenomes. The plastid genomes (plastomes) of both species possess typical quadripartite structures observed across most plants, with sizes of 161,545 bp and 161,230 bp. The overall average GC content of the plastomes of both species was 36.6%. The comparative analysis of the plastome and mitogenome revealed that 12 mitogenome DNA fragments share similar sequences with in the repeat regions of the corresponding plastomes, suggesting a past transfer of repeat regions into the mitogenome. Additionally, the mitogenomes of the two Typha species exhibited high sequence conservation with several syntenic blocks.Phylogenetic analysis of the organellar genomes of the two Typha species and 10 related species produced congruent phylogenetic trees. The availability of these organellar genomes from two Typha species provide valuable genetic resources for studying the evolution of Typhaceae and will improve taxonomic classifications within the family.

    Keywords: Organellar genomes, phylogeny, comparative analysis, Typha, Synteny

    Received: 22 Aug 2024; Accepted: 18 Nov 2024.

    Copyright: © 2024 Soe, Kong, Nie, Wang, Peng, Wu and Tembrock. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Zhiqiang Wu, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
    Luke R Tembrock, Department of Agricultural Biology, Colorado State University, Fort Collins, CO, United States, Colorado State, United States

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