AUTHOR=Wang Xiaoxia , He Lulu , Ullah Haseen , Shi Xiaopeng , Hou Jingyu , Liu Yadong , Liu Yang , Xue Liu , He Baohua , Duan Jie 

TITLE=Combined effects of mixing ratios and tree size: how do mixed forests respond to climate and drought events?

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1477640

DOI=10.3389/fpls.2024.1477640

ISSN=1664-462X

ABSTRACT=<p>Although the relationship between biodiversity and ecosystem functionality (BEF) has been studied comprehensively, how the mixing ratio of tree species in mixed forests affects the response of trees to climate and drought remains an unexplored and rather unknown question. Hence, we established tree-ring chronologies for <italic>Pinus tabuliformis</italic> Carr. (P) and <italic>Quercus variabilis</italic> Blume. (Q) mixed forests with different mixing ratios. In the temperate region of China, we investigated three mixing ratios: 90% P and 10% Q (P9Q1), 60% P and 40% Q (P6Q4), and 20% P and 80% Q (P2Q8). We collected tree ring samples using three tree size categories: dominant, intermediate, and suppressed trees. We explored the climate sensitivity of these trees and their drought tolerance indices–resilience (Rs), resistance (Rt), and recovery (Rc) under two drought conditions: short-term drought (1993 drought) and long-term drought (1999-2015 drought). P6Q4 made <italic>P. tabuliformis</italic> more sensitive to the Palmer drought severity index (PDSI) from the previous year than the other two ratios. The effect of the mixing ratio on drought response was insignificant under short-term drought in both tree species. Rt, Rc, and Rs of <italic>P. tabuliformis</italic> decreased with an increasing <italic>Q. variabilis</italic>:<italic>P. tabuliformis</italic> ratio in long-term drought. Rt, Rc, and Rs of <italic>Q. variabilis</italic> were the highest in P6Q4. The sensitivity of trees to PDSI varied among classes and was influenced by the mixing ratio. Dominant trees were most sensitive to PDSI in P6Q4 and P2Q8, whereas intermediate and suppressed trees were more sensitive to PDSI in P9Q1. The impact of tree size on drought tolerance indices varied according to drought type and mixing ratio. These findings showed that the mixing ratio has a confounding effect on the drought sensitivity of temperate tree species. Differences in hydrological niches allow <italic>Q. variabilis</italic> to benefit from mixing with <italic>P. tabuliformis</italic>. Mixing with optimal proportion of <italic>P. tabuliformis</italic> maximizes the drought resilience of <italic>Q. variabilis</italic>. Additionally, weakly competitive species (<italic>P. tabuliformis</italic>) do not benefit from mixed forests during prolonged water deficits. This result complements previous arguments that species mixing reduces the biological vulnerability of individuals. This study emphasizes the importance of species selection based on the biological and physiological characteristics of tree species in the afforestation of mixed forests. It highlights the critical role of species mixing ratios in the resistance of mixed forest ecosystems to climate change, which may provide a reference for sustainable forest management.</p>