Skip to main content

ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Abiotic Stress
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1470063
This article is part of the Research Topic Plant-Soil-Microbial Interactions in Arid Areas View all 3 articles

Arbuscular mycorrhizal fungi alleviate Mn phytotoxicity by altering Mn subcellular distribution and chemical forms in Lespedeza davidii

Provisionally accepted
Gao Pan Gao Pan Jiayao Hu Jiayao Hu Zheng zi Zheng zi Wenying Wang Wenying Wang Xinhang Li Xinhang Li Xiaoli Xu Xiaoli Xu Wensheng Liu Wensheng Liu *
  • College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, China

The final, formatted version of the article will be published soon.

    Arbuscular mycorrhizal fungi (AMF) can relieve manganese (Mn) phytotoxicity and promote plant growth under Mn stress, while the roles remains unclear. To reveal the detoxification mechanisms of AMF to plants under stress, Lespedeza davidii inoculated with or without AMF (Glomus mosseae) under different Mn concentrations (0, 1, 5, 10, 20 mmol/L) was cultivated by a pot experiment, and plant biomass, physiological and biochemical characteristics, manganese absorption, subcellular distribution and chemical forms of Mn were examined. The results showed that root biomass, stem biomass, leaf biomass and total individual biomass decreased under high Mn concentrations (above 10 mmol/L), and the inoculated plants had higher biomass than the non-inoculated ones. With the rising Mn concentration, the contents of soluble sugar, soluble protein, free proline, superoxide dismutases (SOD), peroxidases (POD) and catalase (CAT) increased firstly and then decreased, while the malondialdehyde (MDA) content increased. The contents of soluble sugar, soluble protein, free proline, SOD, POD and CAT in the inoculation group were higher than those of the uninoculation group at the Mn concentration of 20 mmol/L. The content of MDA in inoculated plants were lower than that of non-inoculated ones. The inoculation of AMF enriched most of manganese in the root system when compared with the non-mycorrhizal treatment ones. Subcellular distribution of Mn indicated that most of the Mn ions were stored in the cell wall and the vacuoles (the soluble fractions), and the proportion of Mn content in the cell wall components and the vacuole components in leaves in the inoculated group was higher than non-inoculated ones. Furthermore, the proportions of Mn extracted by ethanol and deionized water in the uninoculated group in stems and roots were higher than that of the inoculated group, which suggested that AMF could convert Mn into inactive forms. In conclusion, the present study demonstrated that AMF could improve the resistance of L. davidii to Mn toxicity by increasing the activity of antioxidant enzymes, and altering the subcellular distribution and chemical forms of Mn.

    Keywords: arbuscular mycorrhizal fungi, Manganese stress, Lespedeza davidii, Physiological characteristics, Subcellular distribution, Chemical forms

    Received: 25 Jul 2024; Accepted: 28 Oct 2024.

    Copyright: © 2024 Pan, Hu, zi, Wang, Li, Xu and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Wensheng Liu, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.