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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Bioinformatics
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1469267
Mitochondrial genome insights into spatio-temporal distribution and genetic diversity of Dendrobium hancockii Rolfe (Orchidaceae)
Provisionally accepted- 1 Nanjing Normal University, Nanjing, China
- 2 Jiangsu Provincial Engineering Research Center for Technical Industrialization for Dendrobiums, Nanjing 210023, China, nanjing, China
- 3 Ningbo University, Ningbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, ningbo, China
With its distinctive evolutionary rate and inheritance patterns separate from the nuclear genome, organelle genome analysis has become a prominent focus of current research. Not only is it the first complete circular mitochondrial genome published in the Dendrobium genus, but we've also employed the mitochondrial genome of Dendrobium hancockii Rolfe as a foundation for comparative genomics, genetic diversity, and floristic geography studies. Initially, we constructed a circular mitochondrial map spanning 523,952 bp, containing 40 unique protein-coding genes (PCGs), 37 transfer RNA (tRNA) genes, and 4 ribosomal RNA (rRNA) genes.Additionally, comparative analysis involved mitochondrial genes from 26 land plants, revealing a conserved gene cluster segment, "rpl16-ccmFn-rps3-rps19," within the Dendrobium genus. The mitochondrial genome exhibits a lower point mutation rate and a higher structural variation pattern Simultaneously, we utilized mitochondrial and nuclear genes to explore genetic diversity and structure across 103 samples from 23 wild populations of D. hancockii. The data unequivocally divided D. hancockii into two major groups, supported by evidence from population diversity, genetic structure analysis, principal component analysis (PCA), and phylogenetic trees. The publication of D. hancockii 's complete mitochondrial genome not only fills a crucial gap in our understanding of orchid mitochondrial genomes but also lays the groundwork for subsequent molecular marker research. Furthermore, it reveals the research potential for genetic differentiation, providing insights into the species' evolutionary history and phytogeography from a mitochondrial perspective.
Keywords: Dendrobium hancockii, Mitochondrial Genome, SNP, genetic diversity, Nuclear single copy gene
Received: 23 Jul 2024; Accepted: 01 Oct 2024.
Copyright: © 2024 Hou, Wang, Jiang, Xue, Liu, Niu and Ding. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zhitao Niu, Nanjing Normal University, Nanjing, China
Xiaoyu Ding, Nanjing Normal University, Nanjing, China
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