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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1466956

Effects of bioformulation prototype and bioactive extracts from Agaricus bisporus spent mushroom substrate on controlling Rhizoctonia solani of Lactuca sativa L

Provisionally accepted
Rudy Cignola Rudy Cignola gaia Carminati gaia Carminati Andrea Natolino Andrea Natolino Alessandra Di Francesco Alessandra Di Francesco *
  • University of Udine, Udine, Italy

The final, formatted version of the article will be published soon.

    Food security and waste management represent among the main challenges those to be addressed in the near future. The use of bioformulations and bioactive compounds obtained from agricultural wastes could represent some of the solutions for the management of soil-born pathogens. In the present study, Aureobasidium pullulans strain AP1, tested in oil dispersion (OD) formulation prototype and bio-extracts (HWE, hot water extract and WWE, warm water extract) derived from spent mushroom substrate (SMS) of Agaricus bisporus, were tested as sustainable strategies to manage Rhizoctonia solani of lettuce. By in vitro assays, AP1OD at 600 mg L -1 displayed an inhibition by 57% of pathogen mycelial growth and the SMS extract WWE (40 °C) showed a growth stimulation of lettuce seedling by 27%. In vivo assays, AP1OD formulation used against R. solani reduced by 66.6% the soil-borne pathogen incidence on lettuce plants and both bio-extracts significantly stimulated lettuce leaves and roots growth (>200%). AP1OD formulation and HWE treatments increased the lettuce genes expression levels (ggps, hppd pdx1) mainly imputed to plant antioxidant potential, vitamin E and vitamin B6 biosynthesis. The present study reported the potential of a new formulation and two bio-extracts, derived from an agricultural waste, to use against R. solani of lettuce respectively with antifungal and biostimulant properties.

    Keywords: Soil-borne disease, Aureobasidium pullulans, Spent mushroom substrates, formulation, plant growth promotion

    Received: 18 Jul 2024; Accepted: 30 Sep 2024.

    Copyright: © 2024 Cignola, Carminati, Natolino and Di Francesco. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Alessandra Di Francesco, University of Udine, Udine, Italy

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.