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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1466857

Genome wide association study reveals new genes for resistance to striped stem borer in rice (Oryza sativa L.)

Provisionally accepted
Xing Xiang Xing Xiang 1Shuhua Liu Shuhua Liu 1Yuewen He Yuewen He 2Deqiang Li Deqiang Li 1Danso Ofori Andrews Danso Ofori Andrews 1Abdul Ghani Kandhro Abdul Ghani Kandhro 1Tengda Zheng Tengda Zheng 1Xiaoqun Yi Xiaoqun Yi 1Ping Li Ping Li 1Fu Huang Fu Huang 1Aiping Zheng Aiping Zheng 1*
  • 1 Sichuan Agricultural University, Ya'an, China
  • 2 Guangan Vocational & Technical College, Guangan, China

The final, formatted version of the article will be published soon.

    Rice is one of the most important food crops in the world and is important for global food security. However, damage caused by striped stem borer (SSB) seriously threatens rice production and can cause significant yield losses. The development and use of resistant rice varieties or genes is currently the most effective strategy for controlling SSB. We genotyped 201 rice samples using 2849855 high-confidence single nucleotide polymorphisms (SNPs). We conducted a genome-wide association study (GWAS) based on observed variation data of 201 rice cultivars resistant to SSB. We obtained a quantitative trait locus (QTL)-qRSSB4 that confers resistance to SSB. Through annotation and analysis of genes within the qRSSB4 locus, as well as qRT-PCR detection in resistant rice cultivars, we ultimately selected the candidate gene LOC_Os04g34140 (named OsRSSB4) for further analysis. Next, we overexpressed the candidate gene OsRSSB4 in Nipponbare through transgenic methods, resulting in OsRSSB4 overexpressing lines (OsRSSB4OE). In addition, we evaluated the insect resistance of OsRSSB4OE lines using wild type (Nipponbare) as a control. The bioassay experiment results of live plants showed that after 20 days of inoculation with SSB, the withering heart rate of OsRSSB4OE-34 and OsRSSB4OE-39 lines was only 8.3% and 0%, with resistance levels of 1 and 0, respectively; however, the withering heart rate of the wild-type reached 100%, with a resistance level of 9. The results of the in vitro stem bioassay showed that, compared with the wild-type, the average corrected mortality rate of the SSB fed on the OsRSSB4OE line reached 94.3%, and the resistance reached a high level. In summary, we preliminarily confirmed that OsRSSB4 positively regulates the defense of rice against SSB. This research findings reveal new SSB resistance gene resources, providing an important genetic basis for SSB resistance breeding in rice crops.

    Keywords: rice, striped stem borer, GWAS, Resistance genes, SNP

    Received: 18 Jul 2024; Accepted: 27 Aug 2024.

    Copyright: © 2024 Xiang, Liu, He, Li, Andrews, Ghani Kandhro, Zheng, Yi, Li, Huang and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Aiping Zheng, Sichuan Agricultural University, Ya'an, China

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