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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Genetics, Epigenetics and Chromosome Biology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1465514

H3K4me3 changes occur in cell wall genes during the development of Fagopyrum tataricum morphogenic and non-morphogenic calli

Provisionally accepted
Alicja Tomasiak Alicja Tomasiak 1Artur Pinski Artur Pinski 1Anna Milewska-Hendel Anna Milewska-Hendel 1Ignasi Andreu Godall Ignasi Andreu Godall 2Natalia Borowska-Zuchowska Natalia Borowska-Zuchowska 1Joanna Morończyk Joanna Morończyk 1Jordi Moreno-Romero Jordi Moreno-Romero 3*Alexander Betekhtin Alexander Betekhtin 1*
  • 1 University of Silesia in Katowice, Katowice, Poland
  • 2 Centre for Research in Agricultural Genomics, Spanish National Research Council (CSIC), Barcelona, Catalonia, Spain
  • 3 Department of Biochemistry and Molecular Biology, Faculty of Biosciences, Autonomous University of Barcelona, Bellaterra (Barcelona), Spain

The final, formatted version of the article will be published soon.

    Chromatin immunoprecipitation (ChIP) is a powerful technique for studying the interplay between epigenetic modifications and the DNA regions of interest. In combination with sequencing, it can provide the genome-wide enrichment of the specific epigenetic mark, providing vital information on its involvement in the plethora of cellular processes. Here, we describe the genome-wide distribution of H3K4me3 in morphogenic and non-morphogenic callus of Fagopyrum tataricum. Levels of H3K4me3 were higher around the transcription start site, in agreement with the role of this mark in transcriptional activation. The global levels of methylation were higher in the non-morphogenic callus, which indicated increased gene activation compared to the morphogenic callus. We also employed ChIP to analyse the changes in the enrichment of this epigenetic mark on the genes coding for the cell wall proteins in both calli types during the course of the passage. Enrichment of H3K4me3 on cell wall genes was specific for callus type and particular gene expression,, suggesting that the role of this mark in the cell wall remodelling is complex, in agreement with regulation of the cell-wall components in the processes related to dedifferentiation and redifferentiation -leading to the acquisition of the morphogenic potential. This intricacy on the cell wall composition was supported by the immunohistochemical analysis of the cell wall epitopes' distribution of pectins and extensins.Together, these data give a novel insight into the involvement of H3K4me3 in the regeneration processes in F. tataricum in vitro callus tissue culture.

    Keywords: Callus, Cell Wall, ChIP-sequencing, Fagopyrum tataricum, histone modification, Morphogenic, Native chromatin immunoprecipitation, non-morphogenic PLoS One 12

    Received: 16 Jul 2024; Accepted: 05 Sep 2024.

    Copyright: © 2024 Tomasiak, Pinski, Milewska-Hendel, Andreu Godall, Borowska-Zuchowska, Morończyk, Moreno-Romero and Betekhtin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jordi Moreno-Romero, Department of Biochemistry and Molecular Biology, Faculty of Biosciences, Autonomous University of Barcelona, Bellaterra (Barcelona), 08193, Spain
    Alexander Betekhtin, University of Silesia in Katowice, Katowice, Poland

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.