Cai Hui Cai
1,2*
He Shu Zhang
2
Bo Xu
1,2*The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1464747
Transcriptomics and metabolomics analyses reveal pollen abortion mechanism in alfalfa early stage male sterile lines
Provisionally accepted- 1 College of Forestry and Grassland, Jilin Agricultural University, Changchun, China
- 2 Jilin Provincial Key Laboratory of Tree ang Grass Genetics and Breeding,College of Forestry and Grassland Science,Jilin Agricultural University,Changchun, Changchun, Hebei Province, China
- 3 Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (CAAS), Changchun, Jilin Province, China
This study investigates early-stage anther development in cytoplasmic male sterile (CMS) alfalfa lines (MSJN1A) in relation to the isotypic maintainer line (MSJN1B).Histological analyses revealed abnormal degradation of tapetal cells post-meiosis in the CMS line. Notably, during the early mononuclear stage, the central vacuoles in the microspores were absent, leading to evident pollen abortion. These findings suggest that pollen abortion in the CMS line is associated with the delayed disintegration of the tapetum and structural anomalies in microspore vacuoles. Non-targeted metabolome sequencing revealed 401 and 405 metabolites at late tetrad and early mononuclear stages of alfalfa, respectively. Among these, 39 metabolites were consistently upregulated, whereas 85 metabolites were downregulated. Differential analysis revealed 45 and 37 unique metabolites at each respective stage. These metabolites were primarily featured in pathways related to energy, phenylpropane, sucrose and starch, and fatty acid metabolism. Integrated analysis demonstrated that differentially expressed genes and differential metabolites were co-enriched in these pathways.Additionally, quantitative real-time PCR and physiological index analysis confirmed downregulation of key genes involved in anther development, illustrating that changes in upstream gene regulation could significantly impact downstream metabolite levels, ultimately influencing pollen fertility.
Keywords: alfalfa, Cytoplasmic male sterile lines, Transcriptome sequencing, Non-targeted metabolome sequencing, anthers
Received: 15 Jul 2024; Accepted: 22 Nov 2024.
Copyright: © 2024 Cai, Zhang, Yu, Jia, Yu, Xu and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Cai Hui Cai, College of Forestry and Grassland, Jilin Agricultural University, Changchun, China
Jie Wei Yu, Jilin Provincial Key Laboratory of Tree ang Grass Genetics and Breeding,College of Forestry and Grassland Science,Jilin Agricultural University,Changchun, Changchun, Hebei Province, China
Xue Jia, Jilin Provincial Key Laboratory of Tree ang Grass Genetics and Breeding,College of Forestry and Grassland Science,Jilin Agricultural University,Changchun, Changchun, Hebei Province, China
Lan Yu, Jilin Provincial Key Laboratory of Tree ang Grass Genetics and Breeding,College of Forestry and Grassland Science,Jilin Agricultural University,Changchun, Changchun, Hebei Province, China
Bo Xu, College of Forestry and Grassland, Jilin Agricultural University, Changchun, China
Zhe Ying Wang, Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (CAAS), Changchun, Jilin Province, China
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