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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1463300

Integrated eQTL Mapping Approach Reveals Genomic Regions Regulating Candidate Genes of the E8-r3 Locus in Soybean

Provisionally accepted
Jérôme Gélinas Bélanger Jérôme Gélinas Bélanger 1,2Tanya Rose Copley Tanya Rose Copley 2Valerio Hoyos-Villegas Valerio Hoyos-Villegas 1Louise O'Donoughue Louise O'Donoughue 2*
  • 1 McGill University, Montreal, Canada
  • 2 Centre de recherche sur les grains (CÉROM) inc., Saint-Mathieu-de-Beloeil, Canada

The final, formatted version of the article will be published soon.

    Deciphering the gene regulatory networks of critical quantitative trait loci associated with early maturity provides critical information for breeders to unlock soybean's (Glycine max (L.) Merr.) northern potential and expand its cultivation range. The E8-r3 locus is a genomic region regulating the number of days to maturity under constant short-day photoperiodic conditions in two earlymaturing soybean populations (QS15524F2:F3 and QS15544RIL) belonging to maturity groups MG00 and MG000. In this study, we developed a combinatorial expression quantitative trait loci mapping approach using three algorithms (ICIM, IM, and GCIM) to identify the regions that regulate three candidate genes of the E8-r3 locus (Glyma.04G167900/GmLHCA4a, Glyma.04G166300/GmPRR1a, and Glyma.04G159300/GmMDE04). Using this approach, a total of 2,218 trans (2,061 genes) / 7 cis (7 genes) and 4,073 trans (2,842 genes) / 3,083 cis (2,418 genes) interactions were mapped in the QS15524F2:F3 and QS15544RIL populations, respectively.From these interactions, we successfully identified two hotspots (F2_GM15:49,385,092-

    Keywords: expression quantitative trait loci, regulatory hotspots, Early-maturing soybeans, candidate genes, transcription factor, E8-r3 locus, co-expression network analysis

    Received: 11 Jul 2024; Accepted: 21 Oct 2024.

    Copyright: © 2024 Gélinas Bélanger, Copley, Hoyos-Villegas and O'Donoughue. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Louise O'Donoughue, Centre de recherche sur les grains (CÉROM) inc., Saint-Mathieu-de-Beloeil, Canada

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