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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Physiology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1462235
This article is part of the Research Topic Integrative Root Responses to Multiple Environmental Signals for Plant Resilience View all articles

TCA cycle impairment leads to PIN2 internalization and degradation via reduced MAB4 level and ARA6 components in Arabidopsis roots

Provisionally accepted
Xiaomin Song Xiaomin Song 1,2Iwai Ohbayashi Iwai Ohbayashi 1,3Sun Song Sun Song 1Qiuli Wang Qiuli Wang 1Yi Yang Yi Yang 1Mengyuan Lu Mengyuan Lu 1Yuanyuan Liu Yuanyuan Liu 1Shinichiro Sawa Shinichiro Sawa 4Masahiko Furutani Masahiko Furutani 1,4,5*
  • 1 Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China
  • 2 Chinese Academy of Sciences (CAS), Beijing, Beijing, China
  • 3 National Cheng Kung University, Tainan, Tainan County, Taiwan
  • 4 Kumamoto University, Kumamoto, Kumamoto, Japan
  • 5 Fukuoka University, Fukuoka, Japan

The final, formatted version of the article will be published soon.

    The mitochondrial pyruvate dehydrogenase complex (PDC) plays a crucial role in linking the glycolysis pathway and the tricarboxylic acid (TCA) cycle. Previously, we reported that a mutation of MAB1, encoding an E1β subunit of PDC, affects the abundance of auxin efflux carriers PIN-FORMED proteins (PINs) via reduced recycling and enhanced degradation in vacuoles. Here, we further analyzed the effects of TCA cycle inhibition on vesicle trafficking using both the mab1-1 mutant and 3-BP, a TCA cycle inhibitor. Pharmacological and genetic impairment of the TCA cycle induced the aggregated components of ARA6, which is a plant-unique RAB5 GTPase that mediates endosomal trafficking to the plasma membrane. In addition, MAB4, which is an NPH3-like protein that inhibits PIN internalization from the plasma membrane, was severely reduced in 3-BP-treated roots and mab1-1. Furthermore, TCA cycle impairment led to the accumulation of reactive oxygen species in root tips, and treatment with H2O2 reduced MAB4 levels while increasing the internalization of PIN2 from the plasma membrane, and aggregated ARA6-positive compartments. These results suggest that TCA cycle impairment targets PIN proteins for degradation in the vacuole by disrupting both the MAB4-mediated block of internalization and the ARA6-mediated endocytic pathway.

    Keywords: TCA cycle, mtPDC, auxin transport, PIN, NPH3-like, ROS, root development

    Received: 09 Jul 2024; Accepted: 25 Nov 2024.

    Copyright: © 2024 Song, Ohbayashi, Song, Wang, Yang, Lu, Liu, Sawa and Furutani. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Masahiko Furutani, Fukuoka University, Fukuoka, Japan

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