Skip to main content

ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1461798

Chromosomal Analysis of Progenies Between Lilium Intersectional Hybrids and Wild Species Using ND-FISH and GISH

Provisionally accepted
Zhou Mei Zhou Mei 1Zhu Jungang Zhu Jungang 1Xue Yong Xue Yong 1Xu Qian Xu Qian 1Liu Xiaodan Liu Xiaodan 2Zhang Lu Zhang Lu 1Mou Lisha Mou Lisha 1Zeng Lijia Zeng Lijia 1Mengxi Wu Mengxi Wu 1Jiang Beibei Jiang Beibei 1Yin Jia Yin Jia 1Yuanzhi Pan Yuanzhi Pan 1*
  • 1 Sichuan Agricultural University, Ya'an, China
  • 2 Chengdu Botanical Garden (Sichuan), Chengdu, Sichuan Province, China

The final, formatted version of the article will be published soon.

    Intersectional hybrids in lilies possess significant breeding value, however, the lack of complete lily genomes and complex genotypes pose challenges for early identification of lily hybrids. This study aimed to use intersectional hybrid cultivars as female parents and wild lilies as male parents to facilitate early identification of hybrid offsprings and enhance the efficiency and convenience of the process. The nature of the cross combinations using Non-denaturing Fluorescence In Situ Hybridization (ND-FISH) and Genomic In Situ Hybridization (GISH) techniques was investigated. Three novel oligonucleotide probes-Oligo-pTa794, Oligo-pITS and Oligo-telo-were developed for lily chromosome research. Our results demonstrated successful hybridization between wild lilies and intersectional hybrid cultivars, producing a total of 130 hybrid progenies. The combination of ND-FISH and GISH analyses effectively revealed the genomic composition of the hybrid progeny and determined the parental origin of specific chromosomes, enabling more precise, efficient, and convenient identification of hybrid offsprings. This research not only provides significant guidance for lily breeding practices but also offers a valuable reference for the application of ND-FISH and GISH techniques in interspecific hybridization breeding and molecular cytogenetic research across various plant species.

    Keywords: Lilium, Intersectional hybrids, Wild species, Cytogenetics, ND-FISH, GISH, Oligonucleotide Probes

    Received: 09 Jul 2024; Accepted: 01 Oct 2024.

    Copyright: © 2024 Mei, Jungang, Yong, Qian, Xiaodan, Lu, Lisha, Lijia, Wu, Beibei, Jia and Pan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yuanzhi Pan, Sichuan Agricultural University, Ya'an, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.