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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1461357
This article is part of the Research Topic From Classical Breeding to Modern Biotechnological Advancement in Horticultural Crops - Trait Improvement and Stress Resilience, Volume II View all 11 articles

Transcriptome and metabolome profiling unveil the accumulation of chlorogenic acid in autooctoploid Gongju

Provisionally accepted
Li Zhao Li Zhao 1,2,3Yu Cao Yu Cao 1Gaomeng Shan Gaomeng Shan 1Jiayi Zhou Jiayi Zhou 1Xintong Li Xintong Li 1Peng Li Peng Li 1Yansong Wang Yansong Wang 1Songhao An Songhao An 1Ri Gao Ri Gao 1*
  • 1 College of Agriculture, Yanbian University, Yan Ji, China
  • 2 Department of Chemistry, Yanbian University, Yanji, Jilin Province, China
  • 3 Yanbian Academy of Forestry, Jilin, China

The final, formatted version of the article will be published soon.

    Gongju is recognized as one of the four traditional Chinese medicinal herbs, and its main constituents are chlorogenic acid (CGA) and its derivative material. CGA content in autooctoploid Gongju are considerably elevated compared with those in parental tetraploid Gongju at different flowering stages. However, the underlying molecular mechanisms governing the regulation CGA content remain poorly understood.Therefore, we conducted integrated transcriptome and metabolome analyses of different flowering stages in autooctoploid and tetraploid Gongju to elucidate the underlying molecular mechanisms governing CGA biosynthesis. Transcriptome analysis showed that the number of differentially expressed genes in the budding stage (BS), early flowering stage (EF), and full flowering stage (FF) of tetraploid and octoploid Gongju were 3859, 11,211, and 6837, respectively. A total of 563, 466, and 394 differential accumulated metabolites were respectively identified between the bud stages of tetraploid and octoploid Gongju (4BS vs. 8BS), between the early flowering stages of tetraploid and octoploid Gongju (4EF vs. 8EF), and the full flowering stages of tetraploid and octoploid Gongju (4FF vs. 8FF) groups. The integrated analysis of transcriptomics and metabolomics revealed that the expression of pma6460 and mws0178, which are key enzymes involved in the CGA synthesis pathway, increased during the flowering stages of octoploid Gongju relative to that of tetraploid Gongju.CmHQT and CmC3H genes associated with CGA synthesis were higher in octoploid plants than in tetraploid plants at various flowering stages. To investigate the potential regulation of transcription factors involved in CGA synthesis, we analyzed the coexpression of CmC3H and CmHQT with CmMYBs and CmbHLHs. Results indicated that transcription factors, such as CmMYB12 (Cluster-30519.0), CmMYB26 (Cluster-75874.0), CmMYB5 (Cluster-94106.0), CmMYB1 (Cluster-71968.7), CmbHLH62 (Cluster-32024.1), CmbHLH75 (Cluster-62341.0), CmbHLH62 (Cluster-32024.8), CmbHLH75 (Cluster-60210.0), and CmbHLH16 (Cluster-90665.1) play a pivotal role in CGA synthesis regulation. The present study provides novel 3 insights into the molecular mechanisms underlying CGA accumulation in autopolyploid Gongju.

    Keywords: Gongju, Transcriptome, metabolomic, Chlorogenic Acid, transcription factor

    Received: 08 Jul 2024; Accepted: 11 Oct 2024.

    Copyright: © 2024 Zhao, Cao, Shan, Zhou, Li, Li, Wang, An and Gao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Ri Gao, College of Agriculture, Yanbian University, Yan Ji, 133002, China

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