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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Development and EvoDevo
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1461316

Integrated Hormone and Transcriptome Profiles Provide Insight into the Pericarp Differential Development Mechanism Between Mandarin 'Shatangju' and 'Chunhongtangju'

Provisionally accepted
Yongjing Huang Yongjing Huang 1Congyi Zhu Congyi Zhu 1Yibo Hu Yibo Hu 2Sanjiao Yan Sanjiao Yan 3Zhimin Luo Zhimin Luo 3Yanping Zou Yanping Zou 3Wen Wu Wen Wu 1Jiwu Zeng Jiwu Zeng 1*
  • 1 Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China
  • 2 Deqing County Agricultural Technology Promotion Center, Zhaoqing, China
  • 3 Longmen County Agricultural and Rural Comprehensive Service Center, Huizhou, China

The final, formatted version of the article will be published soon.

    Citrus reticulata cv. 'Chunhongtangju' was mutated from Mandarin 'Shatangju', which has been identified as a new citrus variety. Mandarin 'Chunhongtangju' fruits were late-ripening for about two months than Mandarin 'Shatangju'. To understand the pericarp differential development mechanism in Mandarin 'Shatangju' (CK) and 'Chunhongtangju' (LM), hormones and transcriptome profiles of pericarps were performed in different development stages: Young fruit stage (CK1/LM1), Expansion and Turning color stage (CK2), Expansion stage (LM2), Turning color stage (LM3), and Maturity stage (CK3/LM4). In this study, the development of LM was significantly slower, and the maturity was significantly delayed. At the same stage, most hormones in Mandarin 'Chunhongtangju' pericarps were higher than that in 'Shatangju' such as gibberellin A24, cis (+)-12-oxophytodienoic acid, and L-phenylalanine. The deficiency of hormones in latematuring pericarps was mainly manifested in ABA, 12-OH-JA, MeSAG, and ABA-GE. Differences in transcriptome profiles between the two citrus varieties are primarily observed in energy metabolism, signal transduction such as MAPK signaling pathway and plant hormone signaling, and biosynthesis of secondary metabolites. After analyzing the hormones and transcriptome data, we found that the top genes and hormones, such as Cs_ont_5g020040 (transcription elongation factor, TFIIS), Cs_ont_7g021670 (BAG family molecular chaperone regulator 5, BAG5), Cs_ont_2g025760 (40S ribosomal protein S27, Rps27), 5-deoxystrigol, salicylic acid 2-O-βglucosid, and gibberellin A24, contributed significantly to gene transcription and hormone synthesis.This study suggests that the variances of pericarp development between the two varieties are linked to variations in the transcription levels of genes associated with energy and secondary metabolism, signal transduction-related genes. These findings expand our understanding of the complex transcriptional and hormonal regulatory hierarchy during pericarp development.

    Keywords: Mandarin 'Shatangju', Mandarin 'Chunhongtangju', Pericarp, Transcriptome, hormone

    Received: 08 Jul 2024; Accepted: 20 Sep 2024.

    Copyright: © 2024 Huang, Zhu, Hu, Yan, Luo, Zou, Wu and Zeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jiwu Zeng, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China

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