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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1460353
Comparing Performances of Different Statistical Models and Multiple Thresholds Methods in a Nested Association Mapping Population of Wheat
Provisionally accepted- Washington State University, Pullman, Washington, United States
Nested association mapping (NAM) populations emerged as a multi-parental strategy which combines the high statistical power of biparental linkage mapping with greater allelic richness of association mapping. Several statistical models have been developed for marker trait associations (MTAs) in genome-wide association studies (GWAS), which ranges from simple to increasingly complex models. These statistical models vary in their performance for detecting real association with the avoidance of false positives and false negatives. Furthermore, significant threshold methods play an equally important role for controlling spurious associations. In this study, we compared the performance of seven different statistical models ranging from single to multi-locus models on eight different simulated traits with varied genetic architecture for a NAM population of spring wheat (Triticum aestivum L.). The best identified model was further used to identify MTAs for 11 different agronomic and spectral reflectance traits which were collected on the NAM population between 2014 and 2016. The "Bayesian information and linkage disequilibrium iteratively nested keyway (BLINK)" model performed better than all other models observed based on Q-Q plots and detection of real association in a simulated data set. The results from model comparison suggest that BLINK controls both false positives and false negatives under the different genetic architecture of simulated traits. Comparison of multiple significant threshold methods suggests that Bonferroni correction performed superior for controlling false positives and false negatives and complements the performance of GWAS models. BLINK identified 45 MTAs using Bonferroni correction of 0.05 for 11 different phenotypic traits in the NAM population. This study helps identify the best statistical model and significant threshold method for performing association analysis in subsequent NAM population studies.Connecting phenotypes with genotypes provides a vital tool for crop breeding and improvement. The most commonly exploited approaches for genetic mapping include biparental linkage mapping and association mapping. Biparental linkage mapping requires developing a large recombinant population for linkage-based mapping of quantitative trait loci (QTLs) (Lander and Botstein 1989).
Keywords: false positives and false negatives, genome-wide association studies, Nested Association Mapping, single and multi-locus models, Spectral reflectance indices, wheat
Received: 05 Jul 2024; Accepted: 04 Sep 2024.
Copyright: © 2024 Sandhu, Burke, Merrick, Pumphrey and Carter. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Arron Carter, Washington State University, Pullman, 99164, Washington, United States
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