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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Symbiotic Interactions
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1459882
This article is part of the Research Topic Plant-microbes Interactions and Resistance Against Abiotic Stress View all 7 articles
Enhancing Cold Tolerance in Tobacco through Endophytic Symbiosis with Piriformospora indica
Provisionally accepted
Han Li
1
Zhiyao Wang
2*
Yongxu Yu
3*
Weichang Gao
1*
Jingwei Zhu
1*
Heng Zhang
1*
Xiang Li
4*
Yanxia Liu
1*- 1 Guizhou Institute of Tobacco Science, Guiyang, China
- 2 Guizhou University, Guiyang, Guizhou Province, China
- 3 Zunyi Branch of Guizhou Tobacco Company, Zunyi, China
- 4 Guizhou Tobacco Company, Guiyang, Guizhou Province, China
Tobacco, a warm-season crop originating from the Americas, is highly susceptible to cold stress. The utilization of symbiotic fungi as a means to bolster crops' resilience against abiotic stresses has been proven to be a potent strategy. In this study, we investigated the effect of endophytic fungus Piriformospora indica on the cold resistance of tobacco. When exposed to cold stress, the colonization of P.indica in tobacco roots effectively stimulates the activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). This, in turn, reduces the accumulation of reactive oxygen species (ROS), thereby mitigating oxidative damage. Additionally, P. indica elevates the levels of osmolytes, such as soluble sugars, proline, and soluble proteins, thus facilitating the restoration of osmotic balance. Under cold stress conditions, P. indica also induces the expression of coldresponsive genes. Furthermore, this fungus not only enhances photosynthesis in tobacco by stimulating the synthesis of photosynthetic pigments, strengthening Rubisco activity, and elevating PSII efficiency, but also fortifies tobacco's nitrogen assimilation by inducing the expression of nitrate transporter gene and activating enzymes related to nitrogen assimilation. Consequently, this synergistic optimization of nitrogen and carbon assimilation provides a solid material and energetic foundation for tobacco plants to withstand cold stress. Our study demonstrates that a mycorrhizal association between P. indica and tobacco seedlings provides multifaceted protection to tobacco plants against low-temperature stress and offers a valuable insight into how P. indica enhances the cold tolerance of tobacco.
Keywords: piriformospora indica, tobacco cold resistance, Reactive Oxygen Species, osmolytes, Photosynthesis, N assimilation
Received: 05 Jul 2024; Accepted: 08 Oct 2024.
Copyright: © 2024 Li, Wang, Yu, Gao, Zhu, Zhang, Li and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zhiyao Wang, Guizhou University, Guiyang, 550025, Guizhou Province, China
Yongxu Yu, Zunyi Branch of Guizhou Tobacco Company, Zunyi, China
Weichang Gao, Guizhou Institute of Tobacco Science, Guiyang, China
Jingwei Zhu, Guizhou Institute of Tobacco Science, Guiyang, China
Heng Zhang, Guizhou Institute of Tobacco Science, Guiyang, China
Xiang Li, Guizhou Tobacco Company, Guiyang, Guizhou Province, China
Yanxia Liu, Guizhou Institute of Tobacco Science, Guiyang, China
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