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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1459651
This article is part of the Research Topic Enhancing Plant Resistance and Resilience to Phytopathogenic Microbes View all 4 articles

Arbuscular mycorrhizal fungi improve the disease resistance of Lycium barbarum to root rot by activating phenylpropane metabolism

Provisionally accepted
Nan Li Nan Li 1Wei Chen Wei Chen 1Bin Wang Bin Wang 1Chongqing Zhang Chongqing Zhang 1Yupeng Wang Yupeng Wang 1Ruiyun Li Ruiyun Li 1Yuke Yan Yuke Yan 1Jing He Jing He 1,2*
  • 1 College of Forestry, Gansu Agricultural University, Lanzhou, Gansu Province, China
  • 2 Wolfberry Harmless Cultivation Engineering Research Center of Gansu Province, Lanzhou, China, Lanzhou, China

The final, formatted version of the article will be published soon.

    Root rot is one of the common diseases of Lycium barbarum. Pathogens can cause devastating disasters to plants after infecting host plants. This study investigated the effect of arbuscular mycorrhizal fungi (AMF) Rhizophagus intraradices inoculation on phenylpropane metabolism in L. barbarum and evaluated its resistance to root rot. The experiment was set up with AMF inoculation treatments (inoculated or not) and root rot pathogen-Fusarium solani inoculation treatments (inoculated or not).The results showed that AMF was able to form a symbiosis with the root system of L. barbarum, thereby promoting plant growth significantly and increasing plants' resistance to disease stress. The plant height of AMF-colonized L. barbarum increased by 24.83% compared to non-inoculated diseased plants. After inoculation with AMF, the plant defense response induced by pathogen infection was stronger.When the enzyme activity of the leaves reached the maximum after the onset of mycorrhizal L. barbarum, phenylalanine ammonia-lyase, cinnamic acid-4-hydroxylase, and 4-coumaric acid-CoA ligase increased by 3.67%, 31.47%, and 13.61%, respectively, compared with the non-inoculated diseased plants. The products related to the lignin pathway and flavonoid pathway downstream of phenylpropane metabolism such as lignin and flavonoids were also significantly increased by 141.65% and 44.61% compared to nonmycorrhizal diseased plants. The activities of chitinase and β-1,3-glucanase increased by 36.00% and 57.96%, respectively. The contents of salicylic acid and jasmonic acid were also 17.7% and 31.63% higher than those of nonmycorrhizal plants in the early stage of plant growth, respectively. The results indicated that AMF significantly promoted plant growth and enhanced disease resistance by increasing enzyme activities and the production of lignin and flavonoids.字体: (默认)Times New Roman, 小四 设置格式[李南]:Afterwards we isolated and screened the spores using wet sieve decantation-sucrose centrifugation, stained them (Kumar et al., 2008), and observed and counted them under a somatic microscope to finally determine the number of viable spores present in the inoculum.

    Keywords: root rot of Lycium barbarum, Rhizophagus intraradices, Phenylpropane metabolism, Pathogenesis-related protein, Disease Resistance

    Received: 04 Jul 2024; Accepted: 16 Aug 2024.

    Copyright: © 2024 Li, Chen, Wang, Zhang, Wang, Li, Yan and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jing He, College of Forestry, Gansu Agricultural University, Lanzhou, 730070, Gansu Province, China

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