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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1458330
This article is part of the Research Topic Plant Parasitic Nematode–Host Interactions: Mechanisms and Exploitative Management Strategies View all 9 articles

Research on the mechanism of Bacillus velezensis A-27 in enhancing the resistance of red kidney beans to soybean cyst nematode based on TMT proteomics analysis

Provisionally accepted
Yi Hu Yi Hu 1Yibing Ma Yibing Ma 1Liyi Wang Liyi Wang 1*Qingqing Luo Qingqing Luo 1*Zengqi Zhao Zengqi Zhao 2*Jianming Wang Jianming Wang 1Yumei Xu Yumei Xu 1*
  • 1 Shanxi Agricultural University, Jinzhong, China
  • 2 Manaaki Whenua – Landcare Research, Auckland, New Zealand

The final, formatted version of the article will be published soon.

    Soybean cyst nematode (SCN) poses a significant challenge to red kidney beans cultivation, resulting in yield losses and quality deterioration. This study investigates the molecular mechanisms using Tandem Mass Tag (TMT) based proteomics technology to explore how the plant growth-promoting rhizobacterium (PGPR) Bacillus velezensis A-27 enhances the resistance of red kidney beans against SCN.The results revealed that out of 1,374 differentially expressed proteins (DEPs) in the red kidney beans roots, 734 DEPs were upregulated and 640 DEPs were downregulated in the A-27 + J2 vs J2 treatment group. KEGG analysis revealed that 14 DEPs were involved in the α-LeA metabolic pathway, crucial for the biosynthesis of jasmonic acid (JA) in plants. Quantitative real-time PCR (qRT-PCR) confirmed the upregulation of 4 key genes (PLA1, AOS, AOC, ACX) in the JA biosynthesis pathway, while enzyme-linked immunosorbent assay (ELISA) demonstrated a significant increase in JA content in the roots. The study demonstrates that B. velezensis A-27 stimulates induced systemic resistance (ISR) in red kidney beans, and induce JA biosynthesis by regulating the expression of key enzymes in the α-LeA metabolic pathway. This enhances the plant's defense against SCN, providing a theoretical foundation for the potential use of B. velezensis A-27 as a biocontrol agent for managing SCN in leguminous crops.

    Keywords: Red kidney bean, SCN, Bacillus velezensis A-27, JA biosynthesis, TMT, induced systemic resistance (ISR)

    Received: 02 Jul 2024; Accepted: 30 Aug 2024.

    Copyright: © 2024 Hu, Ma, Wang, Luo, Zhao, Wang and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Liyi Wang, Shanxi Agricultural University, Jinzhong, China
    Qingqing Luo, Shanxi Agricultural University, Jinzhong, China
    Zengqi Zhao, Manaaki Whenua – Landcare Research, Auckland, New Zealand
    Yumei Xu, Shanxi Agricultural University, Jinzhong, China

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