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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1457437

Genome-wide linkage mapping of Fusarium crown rot in common wheat (Triticum aestivum L.)

Provisionally accepted
Faji Li Faji Li 1,2*Can Guo Can Guo 3Qi Zhao Qi Zhao 4Weie Wen Weie Wen 5Shengnan Zhai Shengnan Zhai 2Xinyou Cao Xinyou Cao 2Cheng Liu Cheng Liu 2Dungong Cheng Dungong Cheng 2Jun Guo Jun Guo 2Yan Zi Yan Zi 2Aifeng Liu Aifeng Liu 2Jianmin Song Jianmin Song 2Jianjun Liu Jianjun Liu 2Jindong Liu Jindong Liu 6Haosheng Li Haosheng Li 2
  • 1 Crop Research Institute, Shandong Academy of Agricultural Sciences, Ji'nan, China
  • 2 Shandong Academy of Agricultural Sciences, Jinan, Shandong Province, China
  • 3 Shangqiu Academy of Agriculture and Forestry Sciences, Shangqiu, Henan Province, China
  • 4 Yantai University, Yantai, Shandong Province, China
  • 5 Zunyi Medical University, Zunyi, Guizhou Province, China
  • 6 Chinese Academy of Agricultural Sciences (CAAS), Beijing, Beijing Municipality, China

The final, formatted version of the article will be published soon.

    Abstract: Fusarium crown rot (FCR) is a severe soil-borne disease that globally affects wheat and leads to significant yield reductions. Identifying loci associated with resistance to FCR and developing corresponding markers are essential for the breeding of resistant wheat varieties. In this study, we evaluated the resistance to FCR in a recombinant inbred line (RIL) population originated from Gaocheng 8901 and Zhoumai 16 across four environments. The RILs and their parents were genotyped by wheat 90K single-nucleotide polymorphism (SNP) array. We have identified a total of five quantitative trait loci (QTLs) related to FCR resistance: QFCR.caas-3AL, QFCR.caas-3DL, QFCR.caas-5BL, QFCR.caas-6BS, and QFCR.caas-7DS. These QTLs accounted for 4.6% to 12.8% of the phenotypic variance, respectively. Notably, QFCR.caas-5BL and QFCR.caas-6BS had been previously detected, whereas QFCR.caas-3AL, QFCR.caas-3DL and QFCR.caas-7DS are novel loci. The favorable alleles of QFCR.caas-3DL and QFCR.caas-5BL were contributed by Zhoumai 16, while the favorable alleles for QFCR.caas-3AL, QFCR.caas-6BS, and QFCR.caas-7DS were originated from Gaocheng 8901. Additionally, this study identified seven candidate genes that encode disease resistance proteins, the BTB/POZ domains, peroxidase activity, and leucine-rich repeat receptor-like protein kinase. Furthermore, we developed and validated two kompetitive allele-specific PCR (KASP) markers, Kasp_3AL_FCR (QFCR.caas-3AL) and Kasp_5BL_FCR (QFCR.caas-5BL), in a natural population of 202 wheat varieties. This study contributes new genetic insights and provides new stable loci and breeding available KASP markers to enhance FCR resistance in common wheat.

    Keywords: Common wheat, Fusarium crown rot (FCR), Molecular marker-assisted selection, Quantitative Trait Loci (QTL), Kompetitive allele-specific PCR (KASP)

    Received: 30 Jun 2024; Accepted: 03 Oct 2024.

    Copyright: © 2024 Li, Guo, Zhao, Wen, Zhai, Cao, Liu, Cheng, Guo, Zi, Liu, Song, Liu, Liu and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Faji Li, Crop Research Institute, Shandong Academy of Agricultural Sciences, Ji'nan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.