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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1455219

Identification of a novel mutation in the OsMRP5 gene in low phytate Basmati rice mutant and development of CAPS marker for Marker-Assisted Breeding

Provisionally accepted
Zia- ul- Qamar Zia- ul- Qamar 1*Muhammad Uzair Muhammad Uzair 2Amjad Hameed Amjad Hameed 1Syed Adeel Zafar Syed Adeel Zafar 3Xueyong Li Xueyong Li 2
  • 1 Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan
  • 2 National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, Beijing Municipality, China
  • 3 Department of Botany and Plant Sciences, College of Natural and Agricultural Sciences, University of California, Riverside, Riverside, California, United States

The final, formatted version of the article will be published soon.

    Low phytate level is a desirable trait because it promotes mineral bioavailability and thus offers a solution to tackle mineral deficiencies. The objectives of the present study were to characterize low phytate (lpa) Basmati rice mutants for the identification of novel mutations in target gene(s) and to develop a PCR-based CAPS (cleaved amplified polymorphic sequence) marker for low phytate Basmati rice. For this purpose, cultivar Super Basmati (Q4) was irradiated with gamma rays ( 60 Co source) and three mutants named Q1 (lpa-5-9), Q2 (lpa-9-13), and Q3 (lpa-59-14) were isolated. Four genes previously been reported for the low phytic acid trait in rice were sequenced in these mutants and no mutation was observed in Q1 and Q2. However, in Q3 (lpa14) mutant a novel mutation in OsMRP5 gene (LOC_Os03g04920`) was detected. Sequence analysis displayed a substitution in the first exon of OsMRP5 at position 1142 bp resulting in the amino acid change from glycine (Gly) to alanine (Ala) at position 381a.a. To facilitate low-phytate breeding program, CAPS marker was developed to confirm this mutation site using the restriction digestion by AluI restriction enzyme. After enzyme digestion, Q3 produces four bands (32, 220, 154, and 32 bp) while Q4 (parent cultivar Super Basmati) produces only 3 bands (32, 374, and 32 bp). These results showed that this CAPS marker is 100% linked with this mutation and can be used for future breeding programs. Present findings provided insights in molecular basis of low phytate trait in rice paving the way for developing low-phytate rice varieties through marker-assisted breeding.

    Keywords: Gamma Rays, Bio fortification, Mineral deficiency, Oryza sativa, and phytic acid

    Received: 26 Jun 2024; Accepted: 25 Nov 2024.

    Copyright: © 2024 Qamar, Uzair, Hameed, Zafar and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zia- ul- Qamar, Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan

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