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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1452520
This article is part of the Research Topic Improving Yield and Quality of Cereal Crops: Exploring and Utilizing Genes for Green and Efficient Traits View all 3 articles

Triple gene mutations boost amylose and resistant starch content in rice: Insights from sbe2b/sbe1/OE-Wxa mutants

Provisionally accepted
Xiaoqiong Chen Xiaoqiong Chen *Qiaoling Guo Qiaoling Guo Xiaoli Yang Xiaoli Yang Meng Yuan Meng Yuan Jianguo Song Jianguo Song Hongyan Fu Hongyan Fu Hongyu Zhang Hongyu Zhang Xu Peizhou Xu Peizhou Liao Y. Xiang Liao Y. Xiang Asif Ali Asif Ali Kangxi Du Kangxi Du Xianjun Wu Xianjun Wu
  • Sichuan Agricultural University, Ya'an, China

The final, formatted version of the article will be published soon.

    Previous studies have modified rice's resistant starch (RS) content by mutating single and double genes. These mutations include knocking out or reducing the expression of sbe1 or sbe2b genes, as well as overexpressing Wx a . However, the impact of triple mutant sbe2b/sbe1/OE-Wx a on RS contents remained unknown. Here, we constructed a double mutant with sbe2b/RNAi-sbe1, based on IR36ae with sbe2b, and a triple mutant with sbe2b/RNAi-sbe1/OE-Wx a , based on the double mutant. The results showed that the amylose and RS contents gradually increased with an increase in the number of mutated genes. The triple mutant exhibited the highest amylose and RS contents, with 41.92% and 4.63%, respectively, which were 2-and 5-fold higher than those of the wild type, which had 22.19% and 0.86%, respectively. All three mutants altered chain length and starch composition compared to the wild type. However, there was minimal difference observed among the mutants. The Wx a gene contributed to the improvement of 1000-grain weight and seed-setting rate, in addition to the highest amylose and RS contents. Thus, our study offers valuable insight for breeding rice cultivars with a higher RS content and yields.

    Keywords: Resistant Starch, Amylose, OE-Wx a, Triple mutant, Agronomic trait

    Received: 21 Jun 2024; Accepted: 22 Jul 2024.

    Copyright: © 2024 Chen, Guo, Yang, Yuan, Song, Fu, Zhang, Peizhou, Xiang, Ali, Du and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Xiaoqiong Chen, Sichuan Agricultural University, Ya'an, China

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