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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Proteomics and Protein Structural Biology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1450638

The grapevine ABC transporter B family member 15 (VvABCB15) is a transresveratrol transporter out of grapevine cells

Provisionally accepted
Ascensión Martínez Márquez Ascensión Martínez Márquez 1*Viviana Martins Viviana Martins 2Susana Selles Susana Selles 1Hernâni Gerós Hernâni Gerós 2Purificación Corchete Purificación Corchete 3Roque Bru-Martinez Roque Bru-Martinez 1
  • 1 University of Alicante, Alicante, Spain
  • 2 University of Minho, Braga, Braga, Portugal
  • 3 University of Salamanca, Salamanca, Spain

The final, formatted version of the article will be published soon.

    Stilbenes, particularly trans-resveratrol, play a highly relevant defense role in grapevine as phytoalexin is induced in response to stress. Metabolism and transport of stilbenes can be conveniently investigated in grapevine cell culture since large amounts of trans-resveratrol are accumulated in the extracellular medium upon treatment with the elicitor methylated cyclodextrin, either alone or combined with methyl jasmonate. A proteomic approach on grapevine cell membrane fractions was performed to find trans-resveratrol transporter candidates. The candidate VvABCB15 was functionally characterized. Its stable expression in both yeast and Silybum marianum cells heterologous systems led to increased trans-resveratrol transport in these hosts. Transient expression in Vitis cells showed an enhanced absorbent-or elicitor-assisted accumulation of extracellular trans-resveratrol in VvABCB15-expressing or VvGSTU10/VvABCB15-coexpressing cell suspension cultures. Experiments of transient expression in Vitis cell suspensions using light-switchable stilbene synthase (pHYH::VvSTS3) and VvABCB15 further confirmed the candidate's role as a trans-resveratrol transporter. VvABCB15-YFP fusion proteins in Nicotiana leaf showed localization in the plasma membrane, consistent with a functional role in trans-resveratrol transport. This is the first report providing evidence for the involvement of an ABC transporter B-type, VvABCB15, in trans-resveratrol transport to the extracellular medium of grapevine cells.

    Keywords: ATP binding cassette -ABC-transporter B family member 15, Labelfree proteomics analysis, grapevine cell culture, resveratrol, transformation, transport, Vitis vinifera

    Received: 17 Jun 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Martínez Márquez, Martins, Selles, Gerós, Corchete and Bru-Martinez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Ascensión Martínez Márquez, University of Alicante, Alicante, Spain

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