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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1439776

Biocontrol agents transform the stability and functional characteristics of the grape phyllosphere microenvironment

Provisionally accepted
Tao He Tao He 1,2,3Meng Yang Meng Yang 1Hongyan Du Hongyan Du 4Ronghui Du Ronghui Du 1Yueqiu He Yueqiu He 1Sheng Wang Sheng Wang 1Weiping Deng Weiping Deng 1Yixiang Liu Yixiang Liu 1Xiahong He Xiahong He 1Youyong Zhu Youyong Zhu 1Shusheng Zhu Shusheng Zhu 1Fei Du Fei Du 1*
  • 1 Yunnan Agricultural University, Kunming, China
  • 2 State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan Province, China
  • 3 Key Laboratory of Ago-Biodiversity and Pest Management, Ministry of Education, Yunnan Agricultural University, Kunming, Yunnan Province, China
  • 4 Yunnan Academy of Agricultural Sciences, Kunming, Yunnan Province, China

The final, formatted version of the article will be published soon.

    The spread of grape leaf diseases has a negative impact on the sustainable development of agriculture. Diseases induced by Uncinula necator significantly affect the quality of grapes. Bacillus biocontrol agents have been proven effective in disease management. However, limited research has been conducted on the impact of biocontrol agents on the assembly and potential functions of plant phyllosphere microbial communities. The biocontrol bacteria B. subtilis utilized in this study can significantly reduce the disease index of powdery mildew (p＜0.05); concurrently, it exhibits a lower disease index compared to traditional fungicides. A comprehensive analysis has revealed that biocontrol bacteria have no significant impact on the diversity of phyllosphere fungi and bacteria, while fungicides can significantly reduce bacterial diversity. Additionally, biocontrol agents can increase the complexity of fungal networks and enhance the degree of modularity and stability of the bacterial network. The results also showed that the biocontrol agents, which contained a high amount of B. subtilis, were able to effectively colonize the grapevine phyllosphere, creating a microenvironment that significantly inhibits pathogenic bacteria on grape leaves while enhancing leaf photosynthetic capacity. In conclusion, biocontrol agents significantly reduce the grape powdery mildew disease index, promote a microenvironment conducive to symbiotic microorganisms and beneficial bacteria, and enhance plant photosynthetic capacity. These findings provide a basis for promoting biocontrol agents and offer valuable insights into sustainable agriculture development.

    Keywords: Grape, powdery mildew, Phyllosphere microorganism, B. subtilis,, microorganism network, Functional characteristics

    Received: 28 May 2024; Accepted: 30 Sep 2024.

    Copyright: © 2024 He, Yang, Du, Du, He, Wang, Deng, Liu, He, Zhu, Zhu and Du. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Fei Du, Yunnan Agricultural University, Kunming, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.