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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1436429

Temporal transcriptome and metabolome study revealed molecular mechanisms underlying rose responses to red spider mite infestation and predatory mite antagonism

Provisionally accepted
Yanfei Cai Yanfei Cai 1Ziming Shi Ziming Shi 1Peifei Zhao Peifei Zhao 1Yingjie Yang Yingjie Yang 1Yinshan Cui Yinshan Cui 2Min Tian Min Tian 1Jihua Wang Jihua Wang 1*
  • 1 Other
  • 2 Yunnan Pulis Biotechnology Co. Ltd., Kunming, Yunnan, China, Kunming, Yunnan Province, China

The final, formatted version of the article will be published soon.

    Red spider mite (Tetranychus urticae) infestation (SMI) is a detrimental factor for roses grown indoors. Although predatory mite (Neoseiulus californicus) antagonism (PMA) is often utilized to alleviate SMI damage, little is known about the defensive response of greenhouse-grown roses to SMI and the molecular mechanism by which PMA protects roses. To determine the transcriptome and metabolome responses of roses to SMI and PMA, the leaves of a rose cultivar ("Fairy Zixia/Nightingale") were infested with T.urticae. Analysis of the transcriptome and metabolome revealed that 24 h of SMI exerted the most changes in the expression of defense-related genes and metabolites in rose leaves. KEGG pathway analysis of differentially expressed genes (DEGs) and metabolites revealed that rose responses to SMI and PMA were primarily enriched in pathways such as sesquiterpenoid and triterpenoid biosynthesis, benzoxazinoid biosynthesis, stilbenoid, diarylheptanoid and gingerol biosynthesis, phytosterol biosynthesis, MAPK signaling pathway, phenylpropanoid biosynthesis, and others pathways associated with resistance to biotic stress. Rose reacted to SMI and PMA by increasing the expression of structural genes and metabolite levels in phytosterol biosynthesis, mevalonate (MVA) pathway, benzoxazinoid biosynthesis, and stilbenoid biosynthesis. In addition, PMA caused a progressive recover from SMI, allowing rose to revert to its normal growth state. PMA restored the expression of 190 essential genes damaged by SMI in rose leaves, including transcription factors DRE1C, BH035, MYB14, EF110, WRKY24, NAC71, and MY108. However, after 144 h of PMA treatment, rose responsiveness to stimulation was diminished, and after 192 h, the metabolic levels of organic acids and lipids were recovered in large measure. In conclusion, our results offered insights on how roses coordinate their transcriptome and metabolome to react to SMI and PMA, therefore shedding light on how roses, T. urticae, and N. californicus interact.

    Keywords: Tetranychus urticae, Neoseiulus californicus, rose, Transcriptome, Metabolome, plant

    Received: 22 May 2024; Accepted: 29 Jul 2024.

    Copyright: © 2024 Cai, Shi, Zhao, Yang, Cui, Tian and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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