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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Physiology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1436385

Optimization of substrate formulation for Hericium erinaceus by replacing wood by straw and their effect on enzyme activities

Provisionally accepted
Yang Yang Yang Yang 1*Zhu Lu Zhu Lu 2*Lingyun Liu Lingyun Liu 3*Ziming Ren Ziming Ren 2*Shuang Hu Shuang Hu 4*Yue Wang Yue Wang 2*Shujuan Ji Shujuan Ji 2*Xu Wang Xu Wang 5Zhongwei Du Zhongwei Du 5*Yanni Lui Yanni Lui 2*Yanshen Yu Yanshen Yu 2*
  • 1 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China
  • 2 Other, Changchun, China
  • 3 Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin Province, China
  • 4 Jilin Sericulture Science Research Institute, Changchun, China
  • 5 College of Agriculture, Yanbian University, Yan Ji, China

The final, formatted version of the article will be published soon.

    China is rich in straw resources. The utilization of straw in the cultivation of edible fungi partially resolves the resource conflicts between mushroom cultivation and forest industry and also contributes to environmental protection. In this study, based on the technology of replacing wood by grass, the straw formula for mycelial culture of Hericium erinaceus was optimized with Simplex-lattice method commonly used in mixture design. By measuring the growth rate and the activity of lignocellulose degrading enzymes of mycelia in different formulations, and further combining with model optimization, the optimal formulation was screened and validated for mushroom cultivation. In the experiments, different kinds of straw used as the main material showed interaction effects, further affecting the growth rate of mycelia and the activities of laccase, cellulase, and neutral xylanase. The screened optimal formula was composed of 16.3% rice straw, 59.7% cob, 20.0% wheat bran, 2.0% gypsum, 1.0% sucrose, and 1.0% calcium superphosphate. In the mushroom cultivation, 445.69 g of fresh mushroom were obtained and the biological efficiency reached 89.14%. The growth period of the first mushroom was shortened by 7-9 days. Some nutritional components of fruiting bodies, such as crude fats (6.10%), crude proteins (152.02 g/kg), K (19.71 g/kg), P (2.48 g/kg), and Se (6.06 g/kg), were significantly higher than those of the control formula. These above indicators indicated that the screened formula could be applied in the high-yield and high-quality cultivation of H. erinaceus. Our study lays the foundation for expanding cultivation and strains improvement of H. erinaceus, and is conducive to in promoting the rapid development of H. erinaceus industry.

    Keywords: Hericium erinaceus, Substrate formulation, Replacing wood by grass, Simplex-lattice method, enzyme activity

    Received: 22 May 2024; Accepted: 21 Nov 2024.

    Copyright: © 2024 Yang, Lu, Liu, Ren, Hu, Wang, Ji, Wang, Du, Lui and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yang Yang, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China
    Zhu Lu, Other, Changchun, China
    Lingyun Liu, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin Province, China
    Ziming Ren, Other, Changchun, China
    Shuang Hu, Jilin Sericulture Science Research Institute, Changchun, China
    Yue Wang, Other, Changchun, China
    Shujuan Ji, Other, Changchun, China
    Zhongwei Du, College of Agriculture, Yanbian University, Yan Ji, China
    Yanni Lui, Other, Changchun, China
    Yanshen Yu, Other, Changchun, China

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