Skip to main content

ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1435963
This article is part of the Research Topic The Potential of Omics for Understanding and Managing Plant Diseases View all 7 articles

LC-MS based metabolomics identification of natural metabolites against Fusarium oxysporum

Provisionally accepted
Wenjuan Yang Wenjuan Yang 1Sidi Tang Sidi Tang 1Rubing Xu Rubing Xu 2Lu Zhang Lu Zhang 1Zihao Zhou Zihao Zhou 1Yong Yang Yong Yang 1Yanyan Li Yanyan Li 2*Haibo Xiang Haibo Xiang 1*
  • 1 State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Sciences, Hubei University, Wuhan, Hubei Province, China
  • 2 Tobacco Research Institute of Hubei Province, Wuhan, Hebei Province, China

The final, formatted version of the article will be published soon.

    Fusarium is a soil-borne pathogen that poses a serious threat to the quality and yield of hundreds of crops worldwide, particularly tobacco production. Using metabolomics technology, we investigated natural metabolites from disease-conducting soil (DCS) and disease-suppressing soil (DSS) of tobacco rhizosphere as fungicides to control tobacco Fusarium wilt (TFW), which is mainly caused by Fusarium oxysporum.Our preliminary results suggested the antifungal mechanism of these natural metabolites. Metabolomics results showed that the DCS with three different disease grades (G1, G5 and G9 groups) had significantly higher levels of 15, 14 and 233 differential rhizosphere metabolites (DRMs) and significantly lower levels of 72, 152 and 170 DRMs compared to the DSS (G0 group). According to KEGG pathway analysis, these DRMs were found to be enriched in the caffeine metabolism, biosynthesis of phenylpropanoids, galactose metabolism and tyrosine metabolism, etc. Linustatin, scopoletin and phenylpropiolic acid were picked out from these DRMs and found to have suppressive activity against F. oxysporum through correlation analysis and antifungal experiments. The three DRMs showed strong inhibitory effects on the growth and spore germination of F. oxysporum at concentrations of 0.5 mM or higher in each test period. Furthermore, F. oxysporum showed a phobotaxis effect against these three DRMs at concentrations as low as 0.25 mM. Finally, we found that the three DRMs had an inhibitory effect on F. oxysporum by destroying the integrity of the cell membrane and increasing the membrane permeability of F. oxysporum. This study firstly reports the inhibition activity of phenylpropiolic acid and linustatin on F. oxysporum, providing a practical and environmentally friendly method for biocontrol of TFW by using natural fungicides.

    Keywords: Fusarium oxysporum, tobacco Fusarium wilt, Metabolomics, Phenylpropiolic acid, linustatin, scopoletin 1 Introduction

    Received: 21 May 2024; Accepted: 06 Aug 2024.

    Copyright: © 2024 Yang, Tang, Xu, Zhang, Zhou, Yang, Li and Xiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yanyan Li, Tobacco Research Institute of Hubei Province, Wuhan, Hebei Province, China
    Haibo Xiang, State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Sciences, Hubei University, Wuhan, Hubei Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.