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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1435611
This article is part of the Research Topic Virome Analysis for the Identification of Emerging and Re-Emerging Viruses in Plants View all articles

High-throughput sequencing detected a virus-viroid complex in a single pokeweed plant

Provisionally accepted
Giuseppe Parrella Giuseppe Parrella 1*Myeonghwan Kwak Myeonghwan Kwak 2Elisa Troiano Elisa Troiano 1Eui-Joon Kil Eui-Joon Kil 2
  • 1 Institute for Sustainable Plant Protection of the National Research Council (IPSP-CNR), Portici (NA), Italy
  • 2 Department of Plant Medicals, Andong National University, Andong, Republic of Korea

The final, formatted version of the article will be published soon.

    In this study, total RNA high-throughput sequencing (HTS) of a single symptomatic Phytolacca americana plant enabled the obtention of a nearly complete genome of two new isolates of turnip yellows virus (TuYV), named TuYV-ITA1 and TuYV-ITA2 and revealed a mixed infection with a new variant of citrus exocortis viroid (CEVd), named CEVd-ITA1. The TuYV-ITA2 isolate diverged from the known virus isolates of TuYV and showed variability in P0 and P5 readthrough domain. Recombination analysis revealed its recombinant nature between TuYV and an unidentified polerovirus. The putative recombination event was identified in the P5 readthrough domain of the TuYMV-ITA2 isolate. Our results thus represent the first report of TuYV in Italy and some molecular evidence for the possible natural co-existence of TuYV and CEVd in a new natural host for both infectious entities. This study is adding further knowledge about the role of weed plants as virus reservoirs and thus, additional biological and impact studies would be desirable to determine in particular the role of P. americana in the spread of TuYV and if this virus should be considered a new threat for the susceptible Italian crops.

    Keywords: next generation sequencing, Polerovirus, TuYV, CEVd, Phytolacca americana, emerging viruses

    Received: 20 May 2024; Accepted: 29 Jul 2024.

    Copyright: © 2024 Parrella, Kwak, Troiano and Kil. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Giuseppe Parrella, Institute for Sustainable Plant Protection of the National Research Council (IPSP-CNR), Portici (NA), Italy

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