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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1433121
This article is part of the Research Topic Mutational Breeding: From Induced Mutations to Site-Directed Mutagenesis View all 7 articles

Biological responses of an elite centipedegrass [Eremochloa ophiuroides (Munro) Hack.] cultivar (Ganbei) to Carbon Ion Beam Irradiation

Provisionally accepted
Yuan Zhang Yuan Zhang 1Haoran Wang Haoran Wang 1Yan Du Yan Du 2Ling Zhang Ling Zhang 1Xiaohui Li Xiaohui Li 1Hailin Guo Hailin Guo 1Jianxiu Liu Jianxiu Liu 1Libin Zhou Libin Zhou 2Xin Xu Xin Xu 1Jianjian Li Jianjian Li 1*
  • 1 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China
  • 2 Institute of Modern Physics, Chinese Academy of Sciences (CAS), Lanzhou, Gansu Province, China

The final, formatted version of the article will be published soon.

    Carbon ion beam irradiation (CIBI) is a highly efficient mutagenesis for generating mutations that can be used to expand germplasm resources and create superior new germplasm. The study investigated the effects of different doses of CIBI (50 Gy, 100 Gy, 150 Gy, 200 Gy and 300 Gy) on seed germination and seedling survival, seedling morphological and physiological traits of an elite centipedegrass cultivar Ganbei. The results showed that irradiation greater than 50 Gy cause inhibition of seed germination, and the semi-lethal dose (LD50) is around 90 Gy for CIBI treated seeds of Ganbei. A carbon ion beam-mutagenized centipedegrass population was generated from Ganbei, with irradiation dosages from 50 Gy to 200 Gy. More than ten types of phenotypic variations and novel mutants with heritable tendencies mainly including putative mutants of stolon number, length and diameter, of internode length, of leaf length and width, of leaf chlorophyll content, of stolon growth rate, of aboveground tissue dry weight, of sward height were identified.While the total sugar content of the plants from irradiated seeds showed no obvious change in all treatments as compared to the control, the crude protein content displayed significant reduction at a

    Keywords: Centipedegrass, carbon ion beam, Morphological traits, Physiological and biochemical characteristics, SSR analysis

    Received: 15 May 2024; Accepted: 02 Sep 2024.

    Copyright: © 2024 Zhang, Wang, Du, Zhang, Li, Guo, Liu, Zhou, Xu and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jianjian Li, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.