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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Proteomics and Protein Structural Biology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1431164

Comparative proteomic analysis provides insights into wood formation in immature xylem at different ages in Eucalyptus urophylla × Eucalyptus grandis

Provisionally accepted
Guo Liu Guo Liu 1,2Guowu Zhang Guowu Zhang 1*Zhihua Wu Zhihua Wu 1*Xiuhua Shang Xiuhua Shang 1*Chubiao Wang Chubiao Wang 3*Wanhong Lu Wanhong Lu 1*Yan Lin Yan Lin 1*Huang Anying Huang Anying 1Jianzhong Luo Jianzhong Luo 1,2*
  • 1 Research Institute of Fast-growing Trees, Chinese Academy of Forestry, Zhanjiang, Guangdong Province, China
  • 2 State Key Laboratory of Tree Genetics and Breeding aboratory, Chinese Academy of Forestry, Beijing, China
  • 3 College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong Province, China

The final, formatted version of the article will be published soon.

    Wood formation is a crucial developmental stage in the life cycle of a woody plant; this process has substantial scientific research implications and practical applications. However, the mechanisms underlying woody plant development, especially the process of wood formation, remain poorly understood. As eucalyptus is one of the fastest growing tree species in the world, understanding the mechanism of wood formation in eucalyptus will greatly promote the development of molecular breeding technology for forest trees. In this study, we investigated the proteomic profile of immature xylem at four different ages of E. urograndis using iTARQ technology. We identified 5236 proteins and 492 differentially abundant proteins (DAPs). The expression profiles of the DAPs corresponding to coding genes associated with wood formation were assessed using qRT-PCR. From the different expression profiles, it is inferred that the genes encoding kinesin, CDKD3, EXPA13, EXPA2, XTH27, EGases, UGT76E2, LAC, CCoAMT, CesA3, PAL, and CAD may undergo posttranscriptional regulation (PTR). Additionally, the genes encoding EIN2, ETR, MC4-like, and XCP may undergo posttranslational modifications (PTMs). In this study, we investigated changes in wood formationrelated proteins at the protein abundance level in the immature xylem of Eucalyptus urophylla × Eucalyptus grandis, thereby elucidating potential regulatory mechanisms of key proteins involved in eucalyptus wood formation. This study may provide theoretical guidance for further research on molecular breeding techniques and genetic improvement related to the cultivation of rapidly growing and high-quality trees.

    Keywords: Proteomics1, immature xylem2, wood formation3, Gene expression4, Eucalyptus urophylla × Eucalyptus grandis5

    Received: 11 May 2024; Accepted: 10 Oct 2024.

    Copyright: © 2024 Liu, Zhang, Wu, Shang, Wang, Lu, Lin, Anying and Luo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Guowu Zhang, Research Institute of Fast-growing Trees, Chinese Academy of Forestry, Zhanjiang, Guangdong Province, China
    Zhihua Wu, Research Institute of Fast-growing Trees, Chinese Academy of Forestry, Zhanjiang, Guangdong Province, China
    Xiuhua Shang, Research Institute of Fast-growing Trees, Chinese Academy of Forestry, Zhanjiang, Guangdong Province, China
    Chubiao Wang, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong Province, China
    Wanhong Lu, Research Institute of Fast-growing Trees, Chinese Academy of Forestry, Zhanjiang, Guangdong Province, China
    Yan Lin, Research Institute of Fast-growing Trees, Chinese Academy of Forestry, Zhanjiang, Guangdong Province, China
    Jianzhong Luo, Research Institute of Fast-growing Trees, Chinese Academy of Forestry, Zhanjiang, Guangdong Province, China

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