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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Bioinformatics
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1430639
This article is part of the Research Topic Genetically Deciphering the Adaptive Growth and Development of Photosynthetic Organisms: A Holistic Omics Approach View all 6 articles

Organ-specific transcriptional regulation by HFR1 and HY5 in response to shade in Arabidopsis

Provisionally accepted
Ian Kin Yuen Choi Ian Kin Yuen Choi Amit K. Chaturvedi Amit K. Chaturvedi Benny J. Sng Benny J. Sng Vu V. Kien Vu V. Kien In-Cheol Jang In-Cheol Jang *
  • Temasek Life Sciences Laboratory, Singapore, Singapore

The final, formatted version of the article will be published soon.

    Light is crucial for plants and serves as a signal for modulating their growth. Under shade, where red to far-red light ratio is low, plants exhibit shade avoidance responses (SAR). LONG HYPOCOTYL IN FAR-RED 1 (HFR1) and ELONGATED HYPOCOTYL 5 (HY5) are known to be negative regulators of SAR and physically interact with one another. However, transcriptional regulatory network underlying SAR by these two transcription factors has not been explored. Here, we performed organ-specific transcriptome analyses of Arabidopsis thaliana hfr1-5, hy5-215 and hfr1hy5 to identify genes that are co-regulated by HFR1 and HY5 in hypocotyls and cotyledons. Genes co-regulated by HFR1 and HY5 were enriched in various processes related to cell wall modification and chlorophyll biosynthesis in hypocotyls. Phytohormone (abscisic acid and jasmonic acid) and light responses were significantly regulated by HFR1 and HY5 in both organs, though it is more prominent under shade in cotyledons. HFR1 and HY5 also differentially regulate the expression of the cell wall-related genes for xyloglucan endotransglucosylase/hydrolase, expansin, arabinogalactan protein and class III peroxidase depending on the organs. Furthermore, HFR1 and HY5 cooperatively regulate hypocotyl responsiveness to shade through auxin metabolism. Together, our study illustrates the importance of the HFR1-HY5 module in regulating organ-specific shade responses in Arabidopsis.

    Keywords: cell elongation, HFR1, HY5, Hypocotyl, transcription factor, Shade avoidance response

    Received: 10 May 2024; Accepted: 09 Jul 2024.

    Copyright: © 2024 Choi, Chaturvedi, Sng, Kien and Jang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: In-Cheol Jang, Temasek Life Sciences Laboratory, Singapore, Singapore

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