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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1427731
This article is part of the Research Topic Metabolomics in Crop Research – Current and Emerging Methodologies, Volume III View all 4 articles

Metabolomics and quantitative analysis to determine differences in the geographical origins and species of Chinese dragon's blood 1

Provisionally accepted
Xiuting Sun Xiuting Sun 1,2Qing Huang Qing Huang 2Mingsong Wu Mingsong Wu 3Liu He Liu He 2Xiangsheng Zhao Xiangsheng Zhao 2Xinquan Yang Xinquan Yang 2*
  • 1 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
  • 2 Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, haikou, China
  • 3 College of Life Science, Sichuan University, Chengdu, Sichuan Province, China

The final, formatted version of the article will be published soon.

    The aim of this study was to comprehensively analyze the differences in Chinese dragon's blood (CDB), specifically Dracaena cochinchinensis and Dracaena cambodiana, from different geographical origins. Methods: Metabolomic analysis of CDB was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A reliable ultra-performance liquid chromatography method with a photodiode array detector (UHPLC-PDA) was developed and applied for the quantitative analysis of 12 phenolic compounds in 51 batches of samples. Results: A total of 1394 metabolites were detected and annotated, of which 467 were identified as differentially accumulated metabolites. Multivariate analysis revealed that both origin and species had an effect on the composition of CDB, with greater variation between species. 19 phenolic compounds were selected as quality markers to distinguish D. cochinchinensis (Hdsp) from D. cambodiana (Hdca), and oppositin and spinoflavanone a were identified as quality markers to discriminate D. cochinchinensis samples from Hainan (Hdsp) and Guangxi Provinces (Gdc).Quantitative analysis indicated that four phenolic compounds, including loureirin D, 4H-1-benzopyran-4-one,2,3-dihydro-3,5,7-trihydroxy-3-[(4-methoxyphenyl)methyl]-,(R)-, loureirin B, and pterostilbene, showed significant differences between Gdc and Hdsp. Additionally, five phenolic compounds, namely resveratrol, loureirin D, pinostilbene, 4H-1-benzopyran-4-one,2,3-dihydro-3,5,7-trihydroxy-3-[(4methoxyphenyl)methyl]-, (R)-, and loureirin B, exhibited significant differences between Hdsp and Hdca. Conclusion: There are significant differences in the quality of CDB from different geographical origins and species, which lays the foundation for the in-depth development and utilization of different sources of CDB.

    Keywords: Chinese dragon's blood1, Geographical origins2, Species3, metabolomics4, UHPLC-PDA5, Quality control6

    Received: 04 May 2024; Accepted: 29 Aug 2024.

    Copyright: © 2024 Sun, Huang, Wu, He, Zhao and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Xinquan Yang, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, haikou, China

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