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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Biotechnology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1426479

Analysis of plant pararetrovirus promoter sequence/s for developing useful synthetic promoter with enhanced activity in rice, pearl millet, and tobacco plants

Provisionally accepted
Khushbu Kumari Khushbu Kumari 1,2Tsheten Sherpa Tsheten Sherpa 1,2Nrisingha Dey Nrisingha Dey 1*
  • 1 Institute of Life Sciences (ILS), Bhubaneswar, India
  • 2 Regional Centre for Biotechnology (RCB), Faridabad, Haryana, India

The final, formatted version of the article will be published soon.

    Promoter is one of the most important components for many gene-based research as it can fine-tune precise gene expression. Many unique plant promoters have been characterized, but strong promoters with dual expression in both monocot and dicot systems are still lacking. In this study, we attempted to make such a promoter by combining specific domains from monocot-infecting pararetroviral-based promoters Sugarcane Bacilliform Virus (SCBV) and Banana Streak Virus (BSV) to a strong dicotinfecting pararetroviral-based promoter Mirabilis Mosaic Virus (MMV). The generated chimeric promoters, MS, SM, MB, and BM, were tested in monocot and dicot systems and further validated in transgenic tobacco plants. We found that the developed chimeric promoters were species-specific (monocot or dicot), which depended on their respective core promoter (CP) region. Furthermore, with this knowledge, deletion-hybrid promoters were developed and evaluated, which led to the development of a unique dual-expressing promoter, MSD3, with high gene expression efficiency (GUS and GFP reporter genes) in rice, pearl millet, and tobacco plants. We conclude that the MSD3 promoter can be an important genetic tool and will be valuable in plant biology research and application.

    Keywords: Synthetic promoter, Monocot, DiCoT, GUS, Plant pararetrovirus

    Received: 01 May 2024; Accepted: 04 Jul 2024.

    Copyright: © 2024 Kumari, Sherpa and Dey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Nrisingha Dey, Institute of Life Sciences (ILS), Bhubaneswar, India

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.