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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1425759
This article is part of the Research Topic Plant Natural Products: Biosynthesis, Regulation, and Function View all 5 articles
Diterpenoids with Targeting SARS-CoV-2 RdRp from the Roots of Euphorbia fischeriana Steud
Provisionally accepted
Ting Ruan
1
Zhengrui Xiang
1
Yun-Wu Zhang
1
Shirui Fan
1
Juan Ren
1
Qian Zhao
1
Xiaolong Sun
1
Shi-Li Wu
1
Li-Li Xu
1
Miao Qiao
1
Chen-Xu Jing
2
Xiaojiang Hao
1*
Duozhi Chen
1*- 1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences (CAS), Kunming, China
- 2 Research Center of Traditional Chinese Medicine, The Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, Jilin Province, China
Currently, the development of new antiviral drugs against COVID-19 remains of significant importance. In traditional Chinese medicine, the herb Euphorbia fischeriana Steud is often used for antiviral treatment, yet its therapeutic effect against the COVID-19 has been scarcely studied. Therefore, this study focuses on the roots of Euphorbia fischeriana Steud, exploring its chemical composition, antiviral activity against COVID-19, and the underlying basis of its antiviral activity. Ultimately, nine new ent-atisane-type diterpenoid compounds were isolated from Euphorbia fischeriana Steud, named Eupfisenoids A-I (compounds 1-9). The compound of 1 was established as a C-19-degraded ent-atisane-type diterpenoid. During the evaluation of these compounds for their antiviral activity against COVID-19, compound 1 exhibited significant antiviral activity. Furthermore, with the aid of computer virtual screening and microscale thermophoresis (MST) technology, it was found that this compound could directly bind to the RNA-dependent RNA polymerase (RdRp, NSP12) of the COVID-19, a key enzyme in virus replication. This suggests that the compound inhibits virus replication by targeting RdRp. Through this research, not only has our understanding of the antiviral components and material basis of Euphorbia fischeriana Steud been enriched, but also the potential of atisane-type diterpenoid compounds as antiviral agents against COVID-19 has been discovered. The findings mentioned above will provide valuable insights for the development of drugs against COVID-19.
Keywords: Euphorbia fischeriana Steud, diterpenoids, antiviral, SARS-CoV-2 RdRp, Microscale thermophoresis
Received: 30 Apr 2024; Accepted: 01 Jul 2024.
Copyright: © 2024 Ruan, Xiang, Zhang, Fan, Ren, Zhao, Sun, Wu, Xu, Qiao, Jing, Hao and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xiaojiang Hao, State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences (CAS), Kunming, China
Duozhi Chen, State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences (CAS), Kunming, China
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