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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1425700

Overexpression of TaPIP1A Enhances Drought and Salt Stress Tolerance in Arabidopsis: Cross-Species Conservation and Molecular Dynamics

Provisionally accepted
Jie Han Jie Han 1*Wei Zhang Wei Zhang 2Liya Zhi Liya Zhi 3Silong Chen Silong Chen 1Hui Zhao Hui Zhao 1Jun Ji Jun Ji 3Fengzhi Wang Fengzhi Wang 4Junming Li Junming Li 5
  • 1 Hebei University of Science and Technology, Shijiazhuang, China
  • 2 Hebei University of Economics and Business, Shijiazhuang, Hebei Province, China
  • 3 Chinese Academy of Sciences (CAS), Beijing, Beijing, China
  • 4 Cangzhou Academy of Agricultural and Forestry Sciences, Cangzhou, China
  • 5 Hebei Normal University, Shijiazhuang, Hebei Province, China

The final, formatted version of the article will be published soon.

    Understanding the mechanisms underlying plant responses to abiotic stress is crucial for improving crop resilience. This study characterizes the role of TaPIP1A, a member of the plasma membrane intrinsic proteins (PIPs) in wheat, highlighting its critical function in mediating stress responses. We demonstrate that TaPIP1A enhances tolerance to salt and drought stress through specific protein-protein interactions and transcriptional regulation. In TaPIP1A knockout wheat strains, there was a significant decrease in stress tolerance, whereas Arabidopsis plants overexpressing TaPIP1A exhibited marked improvements in survival under similar stress conditions.We also discovered that TaPIP1A interacts with TaPIP2-3 and is regulated by the transcription factor TaWRKY71, which binds to its promoter to activate gene expression. These findings contribute to our understanding of the molecular mechanisms by which PIPs facilitate plant adaptation to environmental stresses, offering new avenues for the development of stress-resistant crop varieties. Our research provides insights into the genetic manipulation of intrinsic membrane proteins to enhance plant stress tolerance, a step forward in agricultural biotechnology.

    Keywords: wheat, TaPIP1A, abiotic stress, Transcriptional regulation, protein-protein interactions, TaWRKY71, Crop resilience, plant stress responses

    Received: 30 Apr 2024; Accepted: 19 Jun 2024.

    Copyright: © 2024 Han, Zhang, Zhi, Chen, Zhao, Ji, Wang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jie Han, Hebei University of Science and Technology, Shijiazhuang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.