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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1420197
This article is part of the Research Topic Innovative Approaches to Agricultural Plant Disease Identification: Integrating Deep Learning into Traditional Methods View all 8 articles

Study of resistance mechanism of Alternaria blight (Alternaria brassicicola (Berk) Sacc.) by biochemical markers in Indian Mustard (Brassica juncea L. Czern. &Coss.)

Provisionally accepted
Anurag Mishra Anurag Mishra 1Nawaz A. Khan Nawaz A. Khan 1Ratnesh Kumar Jha Ratnesh Kumar Jha 2Tamilarasi Murugesh Tamilarasi Murugesh 2Ashutosh Singh Ashutosh Singh 2*
  • 1 Narendra Dev University of Agriculture and Technology, Faizabad, Uttar Pradesh, India
  • 2 Dr. Rajendra Prasad Central Agricultural University, Samastipur, India

The final, formatted version of the article will be published soon.

    Indian mustard (Brassica juncea) is an important oilseed crop in India. Alternaria leaf spot (Alternaria blight) is incited by the fungus Alternaria brassicicola (Berk) Sacc.Alternaria brassicae. It majorly affects crop production leading to a yield loss of up to 70 %. To circumvent this problem, the study of resistant mechanism and identification of biochemical markers is one of the important strategies for its management. In the present study total of 219 genotypes of Indian mustard with check were screened for Alternaria blight in two seasons. Based on AUDPC score Tten consistently performing genotypes were selected for the screening of biochemical and yield attributes under artificial inoculated conditions of Alternaria brassicicola (Berk) Sacc.Alternaria brassicicola. The result showed a negative correlation between disease and yield attributes. The catalase (CAT) activity was significantly increased in resistant genotypes compared to susceptible ones, indicating the crucial role of CAT in the resistance mechanism. Pathogen infection also increases the total protein content and Alternaria resistant genotype showed the highest total soluble protein while susceptible showed the lowest total soluble protein. These ten genotypes were Formatted: Font color: Red categorized by SSI (stress susceptibility index) and Varuna was identified as tolerant and Giriraj as susceptible genotypes for Alternaria brassicicola (Berk) Sacc..Alternaria brassicicola . Varuna and Giriraj were chosen for quantitative analysis of methionine and tryptophan amino acids from seeds by RP-HPLC (Reverse Phase-High Performance Liquid Chromatography) and showed significant differences in the levels of methionine and tryptophan between the Varuna and Giriraj genotypes. Varuna showed higher methionine and tryptophan content compared to the Giriraj genotype. Higher protein content demonstrated an increase in biotic stress-responsive amino acids, such as methionine and tryptophan, suggesting increased resistance to Alternaria diseases in these high-protein genotypes. These amino acids could be used as biochemical markers for Alternaria resistance of mustard.

    Keywords: mustard, Alternaria disease, biochemical, Catalase, Sulfur-containing amino acids

    Received: 19 Apr 2024; Accepted: 19 Jul 2024.

    Copyright: © 2024 Mishra, Khan, Jha, Murugesh and Singh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Ashutosh Singh, Dr. Rajendra Prasad Central Agricultural University, Samastipur, India

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