AUTHOR=Huang Qianglan , Li Xin , Li Qing , Zhong Shengfu , Li Xiuying , Yang Jiezhi , Tan Feiquan , Ren Tianheng , Li Zhi , Suizhuang Yang 

TITLE=Three novel QTLs for FHB resistance identified and mapped in spring wheat PI672538 by bulked segregant analysis of the recombinant inbred line

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1409095

DOI=10.3389/fpls.2024.1409095

ISSN=1664-462X

ABSTRACT=<sec><title>Introduction</title><p><italic>Fusarium</italic> head blight (FHB) has a large influence on both the yield and quality of wheat grain worldwide. Host resistance is the most effective method for controlling FHB, but unfortunately, very few genetic resources on FHB resistance are available; therefore, identifying novel resistance genes or quantitative trait loci (QTLs) is valuable. </p></sec><sec><title>Methods</title><p>Here, a recombinant inbred line (RIL) population containing 451 lines derived from the cross L661/PI672538 was sown in four different environments (2019CZ<sup>a</sup>, 2019CZ<sup>b</sup>, 2021QL and 2021WJ).</p></sec><sec><title>Results</title><p>Five QTLs, consisting of two previously reported QTLs (<italic>FhbL693a</italic> and <italic>FhbL693b</italic>) and three new QTLs (<italic>FhbL693c</italic>, <italic>FhbL693d</italic> and <italic>FhbL693e</italic>), were identified. Further investigation revealed that <italic>FhbL693b</italic>, <italic>FhbL693c</italic> and <italic>FhbL693d</italic> could be detected in all four environments, and <italic>FhbL693a</italic> and FhbL693e were detected only in 2019CZb and 2021WJ, respectively. Among the QTLs, the greatest contribution (10.5%) to the phenotypic variation effect (PVE) was <italic>FhbL693d</italic> in 2021WJ, while the smallest (1.2%) was <italic>FhbL693e</italic> and <italic>FhbL693a</italic> in 2019CZ<sup>b</sup>. The selection of <italic>5Dindel-4</italic> for <italic>FhbL693d</italic>, <italic>4Aindel-7</italic> for <italic>FhbL693c</italic> and <italic>3Bindel-24</italic> for <italic>FhbL693b</italic> decreased the number of damaged spikelets by 2.1, and a new line resistant to FHB named H140-2 was developed by marker-assisted selection (MAS). </p></sec><sec><title>Discussion</title><p>These results could help to further improve FHB resistance in the future.</p></sec>