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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1407840

DArTseq genotyping facilitates the transfer of 'exotic' chromatin from a Secale cereale x S. strictum hybrid into wheat

Provisionally accepted
Kitti Szőke-Pázsi Kitti Szőke-Pázsi 1Klaudia Kruppa Klaudia Kruppa 2Zuzana Tulpova Zuzana Tulpova 3Balázs Kalapos Balázs Kalapos 1Edina Türkösi Edina Türkösi 1Eszter Gaál Eszter Gaál 1Éva Darkó Éva Darkó 1Mahmoud Said Mahmoud Said 3András Farkas András Farkas 1Péter Kovács Péter Kovács 1László Ivanizs László Ivanizs 1Jaroslav Doležel Jaroslav Doležel 3Timothy Rabanus-Wallace Timothy Rabanus-Wallace 4István Molnár István Molnár 1*Éva Szakács Éva Szakács 1
  • 1 Department of Biological Resources, Agricultural Institute, HUN-REN Centre for Agricultural Research (HUN-REN CAR), Martonvásár, Hungary
  • 2 Department of Biological Resources, Agricultural Institute,, HUN-REN Centre for Agricultural Research (HUN-REN CAR), Martonvásár, Hungary
  • 3 Institute of Experimental Botany, Centre of Plant Structural and Functional Genomics, Olomouc, Olomouc, Czechia
  • 4 School of Agriculture, Food, and Ecosystem Sciences, The University of Melbourne, Melbourne, Australia

The final, formatted version of the article will be published soon.

    Cultivated and wild species of genus rye (Secale) are important but underexploited gene sources for increasing the genetic diversity of bread wheat. Gene transfer is possible via bridge genetic materials derived from intergeneric hybrids. During this process, it is essential to precisely identify the rye chromatin in the wheat genetic background. In the present study, backcross generation BC2F8 from a cross between Triticum aestivum (Mv9kr1) and S. cereanum ('Kriszta', a cultivar from the artificial hybrid of S. cereale and S. strictum) were screened using in situ hybridization (GISH and FISH) and analyzed by DArTseq genotyping in order to select potentially agronomically useful genotypes for prebreeding purposes. Of the 329,267 high-quality short sequence reads generated, 27,822 SilicoDArT and 8,842 SNP markers specific to S. cereanum 1R-7R chromosomes were identified. Heatmaps of the marker densities along the 'Lo7' rye reference pseudomolecules revealed subtle differences between the FISH-and DArTseq-based results. This study demonstrates that the 'exotic' rye chromatin of S. cereanum introgressed into wheat can be reliably identified by high-throughput DArTseq genotyping. The Mv9kr1-'Kriszta' addition and translocation lines presented here may serve as valuable prebreeding genetic materials for the development of stress-tolerant or disease-resistant wheat varieties.

    Keywords: Secale cereanum, Triticum aestivum, DArTseq markers, genotyping, Heatmap, Introgression lines, chromosome rearrangements

    Received: 27 Mar 2024; Accepted: 03 Jul 2024.

    Copyright: © 2024 Szőke-Pázsi, Kruppa, Tulpova, Kalapos, Türkösi, Gaál, Darkó, Said, Farkas, Kovács, Ivanizs, Doležel, Rabanus-Wallace, Molnár and Szakács. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: István Molnár, Department of Biological Resources, Agricultural Institute, HUN-REN Centre for Agricultural Research (HUN-REN CAR), Martonvásár, 2462, Hungary

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.