AUTHOR=Wang Qihui , Liu Ning , Yang Ruirui , Zhang Xuejiao , Wang Ying , Li Yongcai , Prusky Dov , Bi Yang , Han Ye 

TITLE=Essential role of ABA signaling and related transcription factors in phenolic acid and lignin synthesis during muskmelon wound healing

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1404477

DOI=10.3389/fpls.2024.1404477

ISSN=1664-462X

ABSTRACT=<p>Abscisic acid (ABA) is a key phytohormone involved in wound healing in fruits and vegetables, while fluridone (FLD) is its synthetic inhibitor. However, it is unknown whether ABA signaling and downstream transcription factors are involved in the synthesis of phenolic acids and lignin monomers in muskmelon wounds, and the underlying mechanisms. In our study, exogenous ABA promoted endogenous ABA synthesis by increasing the levels of β-carotenoid and zeaxanthin, activating 9-cis-epoxycarotenoid dioxygenase (NCED) and zeaxanthin epoxidase (ZEP), facilitated ABA signaling by increasing the expression levels of protein phosphatases type 2C (<italic>CmPP2C</italic>) and ABA-responsive element binding factors (<italic>CmABF</italic>), upregulated the expression levels of <italic>CmMYB1</italic> and <italic>CmWRKY1</italic>, and ABA induced phenylpropanoid metabolism by activating phenylalanine ammonia-lyase (PAL), 4-coenzyme A ligase (4CL), and cinnamyl alcohol dehydrogenase (CAD), which further increased the synthesis of phenolic acids and lignin monomers in muskmelon wounds during healing. Taken together, exogenous ABA induced phenylpropanoid metabolism and increased the synthesis of phenolic acid and lignin monomer in muskmelon wounds during healing, and may be involved in endogenous ABA synthesis and signaling and related transcription factors.</p>