AUTHOR=Zhou Xueqing , Zhong Ting , Wu Meixiu , Li Qian , Yu Wenlin , Gan Longcai , Xiang Xianyu , Zhang Yunyun , Shi Yaru , Zhou Yuanwei , Chen Peng , Zhang Chunyu 

TITLE=Multiomics analysis of a resistant European turnip ECD04 during clubroot infection reveals key hub genes underlying resistance mechanism

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1396602

DOI=10.3389/fpls.2024.1396602

ISSN=1664-462X

ABSTRACT=<p>The clubroot disease has become a worldwide threat for crucifer crop production, due to its soil-borne nature and difficulty to eradicate completely from contaminated field. In this study we used an elite resistant European fodder turnip ECD04 and investigated its resistance mechanism using transcriptome, sRNA-seq, degradome and gene editing. A total of 1751 DEGs were identified from three time points after infection, among which 7 hub genes including <italic>XTH23</italic> for cell wall assembly and two <italic>CPK28</italic> genes in PTI pathways. On microRNA, we identified 17 DEMs and predicted 15 miRNA-target pairs (DEM-DEG). We validated two pairs (miR395-<italic>APS4</italic> and miR160-<italic>ARF</italic>) by degradome sequencing. We investigated the miR395-<italic>APS4</italic> pair by CRISPR-Cas9 mediated gene editing, the result showed that knocking-out <italic>APS4</italic> could lead to elevated clubroot resistance in <italic>B. napus</italic>. In summary, the data acquired on transcriptional response and microRNA as well as target genes provide future direction especially gene candidates for genetic improvement of clubroot resistance on Brassica species.</p>