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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Systematics and Evolution
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1396389

Comparative Analysis of the Complete Mitogenomes of Camellia sinensis var. sinensis and C. sinensis var. assamica Provide Insights into Evolution and Phylogeny Relationship

Provisionally accepted
Li Li Li Li 1*Xiangru Li Xiangru Li 1Yun Liu Yun Liu 1,2Junda Li Junda Li 1,3Xiaoyun Zhen Xiaoyun Zhen 1*Yu Huang Yu Huang 1*Jianghua Ye Jianghua Ye 1Li Fan Li Fan 1*
  • 1 College of Tea and Food Science, Wuyi University, Wuyishan, Fujian Province, China
  • 2 Wuyi University, Wuyishan, Fujian Province, China
  • 3 College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China

The final, formatted version of the article will be published soon.

    Among cultivated tea plants (Camellia sinensis), only four mitogenomes for C. sinensis var. assamica (CSA) have been reported so far but none for C. sinensis var. sinensis (CSS). Here, two mitogenomes of CSS (CSSDHP and CSSRG) have been sequenced and assembled using a combination of Illumina and Nanopore data for the first time. Comparison between CSS and CSA mitogenomes revealed a huge heterogeneity. The number of the repetitive sequences was proportional to the mitogenome size and the repetitive sequences dominated the intracellular gene transfer segments (accounting for 88.7%- 92.8% of the total length). Predictive RNA editing analysis revealed that there might be significant editing in NADH dehydrogenase subunit transcripts. Codon preference analysis showed a tendency to favor A/T bases and T was used more frequently at the third base of the codon. ENc plots analysis showed that the natural selection play an important role in shaping the codon usage bias, and Ka/Ks ratios analysis indicated Nad1 and Sdh3 genes may have undergone positive selection. Further, phylogenetic analysis shows that six C. sinensis clustered together, with the CSA and CSS forming two distinct branches, suggesting two different evolutionary pathway. Altogether, this investigation provided an insight into evolution and phylogeny relationship of C. sinensis mitogenome, thereby enhancing comprehension of the evolutionary patterns within C. sinensis species.

    Keywords: Camellia sinensis, Mitochondrial Genome, genome comparison, Codon preference, positive selection, phylogenetic analysis

    Received: 05 Mar 2024; Accepted: 29 Jul 2024.

    Copyright: © 2024 Li, Li, Liu, Li, Zhen, Huang, Ye and Fan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Li Li, College of Tea and Food Science, Wuyi University, Wuyishan, 354300, Fujian Province, China
    Xiaoyun Zhen, College of Tea and Food Science, Wuyi University, Wuyishan, 354300, Fujian Province, China
    Yu Huang, College of Tea and Food Science, Wuyi University, Wuyishan, 354300, Fujian Province, China
    Li Fan, College of Tea and Food Science, Wuyi University, Wuyishan, 354300, Fujian Province, China

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