Skip to main content

ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Biotechnology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1395696

Identification and expression analysis of calcium-dependent protein kinase (CDPK) family in oat (Avena sativa L.) and their functions in response to saline-alkali stresses

Provisionally accepted
Ya-Nan Li Ya-Nan Li 1Chunyan Lei Chunyan Lei 1Qian Yang Qian Yang 1Xiao Yu Xiao Yu 1Siming Li Siming Li 1Yan Sun Yan Sun 1Chunli Ji Chunli Ji 1Chunhui Zhang Chunhui Zhang 1Jinai Xue Jinai Xue 1*Hongli Cui Hongli Cui 2*Runzhi Li Runzhi Li 1*
  • 1 Shanxi Agricultural University, Jinzhong, Shanxi Province, China
  • 2 Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (CAS), Yantai, China

The final, formatted version of the article will be published soon.

    Calcium-dependent protein kinases (CDPKs) serve as calcium ion sensors and play crucial roles in all aspects of plant life cycle. While CDPK gene family has been extensively studied in various plants, there is limited information available for CDPK members in oat, an important cereal crop worldwide. Totally, 60 AsCDPK genes were identified in oat genome and were classified into four subfamilies based on their phylogenetic relationship. The members within each subfamily shared similar gene structure and conserved motifs. Collinearity analysis revealed that AsCDPK gene amplification was attributed to segmental duplication events and underwent strong purifying selection. AsCDPK promoters were predicted to contain cis-acting elements associated with hormones, biotic and abiotic stresses. AsCDPK gene expressions were induced by different salt stresses, exhibiting stress-specific under different salt treatments. Moreover, overexpression of AsCDPK26 gene enhanced salt resistance in C. reinhardtii, a single-cell photoautotrophic model plants. Further analysis revealed a significant correlation between AsCDPK26 and Na + /H + antiporter 1 (p<0.05), suggesting that AsCDPK26 may interact with ion transporter to modulate salt resistance. These results not only provide valuable insights into AsCDPK genes in response to different salt stresses, but also lay the foundation to mine novel candidates for improving salt tolerance in oat and other crops.

    Keywords: oat (Avena sativa L.), Saline-alkali stresses, Calcium-dependent protein kinase (CDPK), Chlamydomonas reinhardtii, genetic transformation, Na Oat (Avena sativa L.), Na

    Received: 04 Mar 2024; Accepted: 18 Sep 2024.

    Copyright: © 2024 Li, Lei, Yang, Yu, Li, Sun, Ji, Zhang, Xue, Cui and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jinai Xue, Shanxi Agricultural University, Jinzhong, Shanxi Province, China
    Hongli Cui, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (CAS), Yantai, China
    Runzhi Li, Shanxi Agricultural University, Jinzhong, Shanxi Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.